BMRB: 7365

Solution structure of Fe65 WW domain

Authors

Kouno, T., Mizuguchi, M., Nozawa, Y., Sugaya, T., Kawano, K.

Assembly

WW domain

Entity

1. WW domain (polymer, Thiol state: not present), 55 monomers, 6098.464 Da Detail

GPLGSDSFWN PNAFETDSDL PAGWMRVQDT SGTYYWHIPT GTTQWEPPGR ASPSQ

Formula weight

6098.464 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.5 %, Completeness: 78.9 %, Completeness (bb): 79.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	78.9 % (487 of 617)	91.8 % (293 of 319)	58.9 % (142 of 241)	91.2 % (52 of 57)
Backbone	79.7 % (252 of 316)	93.6 % (102 of 109)	65.4 % (104 of 159)	95.8 % (46 of 48)
Sidechain	80.9 % (283 of 350)	91.0 % (191 of 210)	65.6 % (86 of 131)	66.7 % (6 of 9)
Aromatic	40.9 % (36 of 88)	79.5 % (35 of 44)	0.0 % (0 of 40)	25.0 % (1 of 4)
Methyl	100.0 % (34 of 34)	100.0 % (17 of 17)	100.0 % (17 of 17)

1. Amyloid beta A4 precursor protein-binding family B member 1

GPLGSDSFWN PNAFETDSDL PAGWMRVQDT SGTYYWHIPT GTTQWEPPGR ASPSQ

Show sample condition

Sample #1

Solvent system 90% H2O, 10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.65mM Fe65 WW domain U-15N, 13C; 10mM phosphate buffer NA; 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	WW domain	[U-13C; U-15N]		0.65 mM
2	phosphate buffer			10 mM
3	D2O			10 %
4	H2O	natural abundance		90 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.65mM Fe65 WW domain U-15N, 13C; 10mM phosphate buffer NA; 100% D2O

#	Name	Isotope labeling	Type	Concentration
5	WW domain	[U-13C; U-15N]		0.65 mM
6	phosphate buffer			10 mM
7	D2O			100 %

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LACS Plot; CA

Referencing offset: -0.2 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.2 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.01 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	internal	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	internal	indirect	0.1013291

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Protein Blocks Logo

Calculated from 25 models in PDB: 2E45, Strand ID: A Detail

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Release date

2009-10-14

Citation

Fe65-PTB2 Dimerization Mimics Fe65-APP Interaction
Kouno, T., Mizuguchi, M., Nozawa, Y., Sugaya, T., Kawano, K.
Front. Mol. Neurosci. (2017), 10, 140-140, PubMed 28553201 , DOI 10.3389/fnmol.2017.00140 , Abstract

Related entities 1. WW domain, : 1 : 16 entities Detail

Interaction partners 1. WW domain, : 3 interactors Detail

Experiments performed 4 experiments Detail