BMRB: 7366

Solution Structure of Putative periplasmic protein: Northest Structural Genomics Target StR106

Authors

Zhang, Q., Liu, G., Wang, H., Nwosu, C., Cunningham, K., Ma, L.C., Xiao, R., Liu, J., Baran, M.C., Swapna, G.V.T., Thomas, B.A., Rost, B., Montelione, G.T., Szypersk, T.

Assembly

Periplasmic protein

Entity

1. Periplasmic protein (polymer, Thiol state: not present), 99 monomers, 10984.44 Da Detail

MKTGYKVMLG ALAFVVTNVY AAEIMKKTDF DKVASEYTKI GTISTTGEMS PLDAREDLIK KADEKGADVV VLTSGQTENK IHGTADIYKK KLEHHHHHH

Formula weight

10984.44 Da

Source organism

Salmonella enterica

Exptl. method

solution NMR

Refine. method

distance geometry, simulated annealing, molecular dynamics, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 82.8 %, Completeness: 73.2 %, Completeness (bb): 69.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	73.2 % (832 of 1136)	80.5 % (470 of 584)	62.3 % (281 of 451)	80.2 % (81 of 101)
Backbone	69.4 % (411 of 592)	81.9 % (167 of 204)	56.9 % (165 of 290)	80.6 % (79 of 98)
Sidechain	78.0 % (496 of 636)	79.7 % (303 of 380)	75.5 % (191 of 253)	66.7 % (2 of 3)
Aromatic	55.0 % (44 of 80)	62.5 % (25 of 40)	47.5 % (19 of 40)
Methyl	78.8 % (93 of 118)	79.7 % (47 of 59)	78.0 % (46 of 59)

1. Putative periplasmic protein

MKTGYKVMLG ALAFVVTNVY AAEIMKKTDF DKVASEYTKI GTISTTGEMS PLDAREDLIK KADEKGADVV VLTSGQTENK IHGTADIYKK KLEHHHHHH

Show sample condition

Sample

Solvent system 90% H2O, 10% D2O, Pressure 1 atm, Temperature 298 K, pH 4.5, Details 1.1 mM protein, 10mM Tris, 100mM NaCl, 0.02% NaN3, 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Periplasmic protein			1.1 mM
2	Tris			10 mM
3	NaCl			100 mM
4	NaN3			0.02 %
5	D2O			10 %
6	H2O	natural abundance		90 %

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LACS Plot; CA

Referencing offset: 0.17 ppm, Outliers: 2 Detail

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Chemical shift reference

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LACS Plot; CB

Referencing offset: 0.17 ppm, Outliers: 2 Detail

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Chemical shift reference

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LACS Plot; HA

Referencing offset: -0.05 ppm, Outliers: 1 Detail

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Chemical shift reference

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Protein Blocks Logo

Calculated from 20 models in PDB: 2NOC, Strand ID: A Detail

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Release date

2009-10-08

Related entities 1. Periplasmic protein, : 1 : 7 entities Detail

Experiments performed 5 experiments Detail