BMRBj - BMREntryMaster

Found 1 Document (1.681 seconds)

BMRB: 7371

2OA4

Solution NMR Structure: Northeast Structural Genomics Consortium Target SiR5

Authors

Liu, J., Wang, L., Rossi, P., Chen, C.X., Nwosu, C., Cunningham, K., Ma, L.-C., Xiao, R., Baran, M.C., Swapna, G.V.T., Acton, T.B., Burkhard, R., Montelione, G.T.

Assembly

SiR5

Entity

1. SiR5 (polymer, Thiol state: not present), 101 monomers, 11455.09 Da Detail

MMFLRKVEGP RSVTLPDGSI MTRADLPPAN TRRWVASRKI AVVRGVIYGL ITLAEAKQTY GLSDEEFNSW VSALAEHGKD ALKVTALKKY RQLLEHHHHH H

Formula weight

11455.09 Da

Source organism

Ruegeria pomeroyi

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.1 %, Completeness: 89.9 %, Completeness (bb): 91.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	89.9 % (1068 of 1188)	89.8 % (553 of 616)	90.0 % (422 of 469)	90.3 % (93 of 103)
Backbone	91.1 % (545 of 598)	91.7 % (187 of 204)	91.2 % (271 of 297)	89.7 % (87 of 97)
Sidechain	89.2 % (611 of 685)	88.8 % (366 of 412)	89.5 % (239 of 267)	100.0 % (6 of 6)
Aromatic	68.8 % (66 of 96)	68.8 % (33 of 48)	67.4 % (31 of 46)	100.0 % (2 of 2)
Methyl	100.0 % (124 of 124)	100.0 % (62 of 62)	100.0 % (62 of 62)

1. SiR5

MMFLRKVEGP RSVTLPDGSI MTRADLPPAN TRRWVASRKI AVVRGVIYGL ITLAEAKQTY GLSDEEFNSW VSALAEHGKD ALKVTALKKY RQLLEHHHHH H

Show sample condition

Sample #1

Solvent system 5% D2O, 95% H2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 1.0mM U-13C, 15N SiR5, 5% D2O, 95% H2O

#	Name	Isotope labeling	Concentration
1	SiR5	[U-13C; U-15N]	1.0 mM
2	NaCl		0.1 mM
3	D2O		5 %
4	H2O	natural abundance	95 %

Sample #2

Solvent system 5% D2O, 95% H2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 1.150mM 5%-13C, 15N SiR5, 5% D2O, 95% H2O

#	Name	Isotope labeling	Concentration
5	SiR5	[U-5% 13C; U-15N]	1.150 mM
6	NaCl		0.1 M
7	D2O		5 %
8	H2O	natural abundance	95 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.15 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.15 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.16 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2OA4, Strand ID: A Detail

Release date

2010-03-08

Related entities 1. SiR5, : 1 : 3 entities Detail

Experiments performed 13 experiments Detail

1 3D_13C-separated_NOESY

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Solvent system 5% D2O, 95% H2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 1.0mM U-13C, 15N SiR5, 5% D2O, 95% H2O

#	Name	Isotope labeling	Concentration
1	SiR5	[U-13C; U-15N]	1.0 mM
2	NaCl		0.1 mM
3	D2O		5 %
4	H2O	natural abundance	95 %

2 3D_15N-separated_NOESY

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Solvent system 5% D2O, 95% H2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 1.0mM U-13C, 15N SiR5, 5% D2O, 95% H2O

#	Name	Isotope labeling	Concentration
1	SiR5	[U-13C; U-15N]	1.0 mM
2	NaCl		0.1 mM
3	D2O		5 %
4	H2O	natural abundance	95 %

3 HCCH-TOCSY

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Solvent system 5% D2O, 95% H2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 1.0mM U-13C, 15N SiR5, 5% D2O, 95% H2O

#	Name	Isotope labeling	Concentration
1	SiR5	[U-13C; U-15N]	1.0 mM
2	NaCl		0.1 mM
3	D2O		5 %
4	H2O	natural abundance	95 %

4 HCCH-COSY

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Solvent system 5% D2O, 95% H2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 1.0mM U-13C, 15N SiR5, 5% D2O, 95% H2O

#	Name	Isotope labeling	Concentration
1	SiR5	[U-13C; U-15N]	1.0 mM
2	NaCl		0.1 mM
3	D2O		5 %
4	H2O	natural abundance	95 %

5 CccoNH-TOCSY

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Solvent system 5% D2O, 95% H2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 1.0mM U-13C, 15N SiR5, 5% D2O, 95% H2O

#	Name	Isotope labeling	Concentration
1	SiR5	[U-13C; U-15N]	1.0 mM
2	NaCl		0.1 mM
3	D2O		5 %
4	H2O	natural abundance	95 %

6 3D-HNCACB

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Solvent system 5% D2O, 95% H2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 1.0mM U-13C, 15N SiR5, 5% D2O, 95% H2O

#	Name	Isotope labeling	Concentration
1	SiR5	[U-13C; U-15N]	1.0 mM
2	NaCl		0.1 mM
3	D2O		5 %
4	H2O	natural abundance	95 %

7 HNcoCACB

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Solvent system 5% D2O, 95% H2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 1.0mM U-13C, 15N SiR5, 5% D2O, 95% H2O

#	Name	Isotope labeling	Concentration
1	SiR5	[U-13C; U-15N]	1.0 mM
2	NaCl		0.1 mM
3	D2O		5 %
4	H2O	natural abundance	95 %

8 HBHAcoNH

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Solvent system 5% D2O, 95% H2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 1.0mM U-13C, 15N SiR5, 5% D2O, 95% H2O

#	Name	Isotope labeling	Concentration
1	SiR5	[U-13C; U-15N]	1.0 mM
2	NaCl		0.1 mM
3	D2O		5 %
4	H2O	natural abundance	95 %

9 HNCA

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Solvent system 5% D2O, 95% H2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 1.0mM U-13C, 15N SiR5, 5% D2O, 95% H2O

#	Name	Isotope labeling	Concentration
1	SiR5	[U-13C; U-15N]	1.0 mM
2	NaCl		0.1 mM
3	D2O		5 %
4	H2O	natural abundance	95 %

10 HNCO

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Solvent system 5% D2O, 95% H2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 1.0mM U-13C, 15N SiR5, 5% D2O, 95% H2O

#	Name	Isotope labeling	Concentration
1	SiR5	[U-13C; U-15N]	1.0 mM
2	NaCl		0.1 mM
3	D2O		5 %
4	H2O	natural abundance	95 %

11 C13_HSQC_noct Stereospecific VL Me assign

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Solvent system 5% D2O, 95% H2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 1.150mM 5%-13C, 15N SiR5, 5% D2O, 95% H2O

#	Name	Isotope labeling	Concentration
5	SiR5	[U-5% 13C; U-15N]	1.150 mM
6	NaCl		0.1 M
7	D2O		5 %
8	H2O	natural abundance	95 %

12 Het-NOE, T1/Tirho

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Solvent system 5% D2O, 95% H2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 1.150mM 5%-13C, 15N SiR5, 5% D2O, 95% H2O

#	Name	Isotope labeling	Concentration
5	SiR5	[U-5% 13C; U-15N]	1.150 mM
6	NaCl		0.1 M
7	D2O		5 %
8	H2O	natural abundance	95 %

13 C13 Aromatic NOESY

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Solvent system 5% D2O, 95% H2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 1.0mM U-13C, 15N SiR5, 5% D2O, 95% H2O

#	Name	Isotope labeling	Concentration
1	SiR5	[U-13C; U-15N]	1.0 mM
2	NaCl		0.1 mM
3	D2O		5 %
4	H2O	natural abundance	95 %

nullKeywords NESG, NMR structure, Northeast Structural Genomics Consortium, Protein Structure Initiative, PSI-2, SiR5, Structural Genomics

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Found 1 Document (1.681 seconds)

BMRB: 7371

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. SiR5, : 1 : 3 entities Detail

Experiments performed 13 experiments Detail

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