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BMRB: 7381

2P0P

Calcium binding protein in the free form

Authors

Zhang, X., Hu, Y., Jin, C.

Assembly

Alr1010 protein

Entity

1. Alr1010 protein (polymer, Thiol state: all free), 126 monomers, 14750.25 Da Detail

MASVERDETR EHRIETEIIV DAEDKEERAM GWYYYLDDTL EFPFMGKWKK KSRKTSTIEE KTVEVLGMAP DDECLKDMYV EVADIGGKDD DVYTAKLSDI EAIDVDDDTQ EAIADWLYWL ARGYKF

Formula weight

14750.25 Da

Source organism

Exptl. method

Refine. method

simulated annealing, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 90.4 %, Completeness (bb): 97.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	90.4 % (1332 of 1474)	88.5 % (675 of 763)	91.4 % (532 of 582)	96.9 % (125 of 129)
Backbone	97.9 % (736 of 752)	98.0 % (251 of 256)	98.1 % (365 of 372)	96.8 % (120 of 124)
Sidechain	85.0 % (716 of 842)	83.6 % (424 of 507)	87.0 % (287 of 330)	100.0 % (5 of 5)
Aromatic	47.1 % (65 of 138)	52.2 % (36 of 69)	38.5 % (25 of 65)	100.0 % (4 of 4)
Methyl	100.0 % (128 of 128)	100.0 % (64 of 64)	100.0 % (64 of 64)

1. Alr1010 protein

MASVERDETR EHRIETEIIV DAEDKEERAM GWYYYLDDTL EFPFMGKWKK KSRKTSTIEE KTVEVLGMAP DDECLKDMYV EVADIGGKDD DVYTAKLSDI EAIDVDDDTQ EAIADWLYWL ARGYKF

Show sample condition

Sample

Solvent system 90% H2O, 10% D2O, Pressure 1 null, Temperature 303 K, pH 7.5, Details 1mM Ccbp U-15N,13C, 20mM Tris, 220mM NaCl,10mM DTT; 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Alr1010_protein	[U-13C; U-15N]		1 mM
2	Tris			20 mM
3	NaCl			220 mM
4	CaCl2			40 mM
5	DTT			10 mM
6	D2O			10 %
7	H2O	natural abundance		90 %

LACS Plot; CA

Referencing offset: -0.32 ppm, Outliers: 1 Detail

LACS Plot; CB

Referencing offset: -0.32 ppm, Outliers: 1 Detail

LACS Plot; HA

Referencing offset: -0.03 ppm, Outliers: 1 Detail

LACS Plot; CO

Referencing offset: -0.05 ppm, Outliers: 4 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2P0P, Strand ID: A Detail

Release date

2015-10-26

Related entities 1. Alr1010 protein, : 1 : 3 : 2 entities Detail

Experiments performed 6 experiments Detail

1 3D_13C-separated_NOESY

Instrument

Bruker AVANCE - 800 MHz

Sample

State isotropic, Solvent system 90% H2O, 10% D2O, Pressure 1 null, Temperature 303 K, pH 7.5, Details 1mM Ccbp U-15N,13C, 20mM Tris, 220mM NaCl,10mM DTT; 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Alr1010_protein	[U-13C; U-15N]		1 mM
2	Tris			20 mM
3	NaCl			220 mM
4	CaCl2			40 mM
5	DTT			10 mM
6	D2O			10 %
7	H2O	natural abundance		90 %

2 3D_15N-separated_NOESY

Instrument

Bruker AVANCE - 800 MHz

Sample

State isotropic, Solvent system 90% H2O, 10% D2O, Pressure 1 null, Temperature 303 K, pH 7.5, Details 1mM Ccbp U-15N,13C, 20mM Tris, 220mM NaCl,10mM DTT; 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Alr1010_protein	[U-13C; U-15N]		1 mM
2	Tris			20 mM
3	NaCl			220 mM
4	CaCl2			40 mM
5	DTT			10 mM
6	D2O			10 %
7	H2O	natural abundance		90 %

3 HNCACB,CBCA(CO)NH

Instrument

Bruker AVANCE - 800 MHz

Sample

State isotropic, Solvent system 90% H2O, 10% D2O, Pressure 1 null, Temperature 303 K, pH 7.5, Details 1mM Ccbp U-15N,13C, 20mM Tris, 220mM NaCl,10mM DTT; 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Alr1010_protein	[U-13C; U-15N]		1 mM
2	Tris			20 mM
3	NaCl			220 mM
4	CaCl2			40 mM
5	DTT			10 mM
6	D2O			10 %
7	H2O	natural abundance		90 %

4 HNCO,HN(CA)CO

Instrument

Bruker AVANCE - 800 MHz

Sample

State isotropic, Solvent system 90% H2O, 10% D2O, Pressure 1 null, Temperature 303 K, pH 7.5, Details 1mM Ccbp U-15N,13C, 20mM Tris, 220mM NaCl,10mM DTT; 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Alr1010_protein	[U-13C; U-15N]		1 mM
2	Tris			20 mM
3	NaCl			220 mM
4	CaCl2			40 mM
5	DTT			10 mM
6	D2O			10 %
7	H2O	natural abundance		90 %

5 HC(C)H-COSY

Instrument

Bruker AVANCE - 800 MHz

Sample

State isotropic, Solvent system 90% H2O, 10% D2O, Pressure 1 null, Temperature 303 K, pH 7.5, Details 1mM Ccbp U-15N,13C, 20mM Tris, 220mM NaCl,10mM DTT; 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Alr1010_protein	[U-13C; U-15N]		1 mM
2	Tris			20 mM
3	NaCl			220 mM
4	CaCl2			40 mM
5	DTT			10 mM
6	D2O			10 %
7	H2O	natural abundance		90 %

6 HC(C)H-TOCSY

Instrument

Bruker AVANCE - 800 MHz

Sample

State isotropic, Solvent system 90% H2O, 10% D2O, Pressure 1 null, Temperature 303 K, pH 7.5, Details 1mM Ccbp U-15N,13C, 20mM Tris, 220mM NaCl,10mM DTT; 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Alr1010_protein	[U-13C; U-15N]		1 mM
2	Tris			20 mM
3	NaCl			220 mM
4	CaCl2			40 mM
5	DTT			10 mM
6	D2O			10 %
7	H2O	natural abundance		90 %

nullKeywords calcium binding