BMRB: 7382

NMR Structure of RRM-2 of Yeast NPL3 Protein

Authors

Deka, P., Bucheli, M., Skrisovska, L., Allain, F.H., Moore, C., Buratowski, S., Varani, G.

Assembly

Nucleolar protein 3

Entity

1. Nucleolar protein 3 (polymer, Thiol state: all free), 87 monomers, 9952.040 Da Detail

KLPAKRYRIT MKNLPEGCSW QDLKDLAREN SLETTFSSVN TRDFDGTGAL EFPSEEILVE ALERLNNIEF RGSVITVERD DNPPPIR

Formula weight

9952.04 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.7 %, Completeness: 87.4 %, Completeness (bb): 94.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	87.4 % (892 of 1021)	90.7 % (487 of 537)	80.3 % (317 of 395)	98.9 % (88 of 89)
Backbone	94.9 % (484 of 510)	96.5 % (166 of 172)	92.6 % (238 of 257)	98.8 % (80 of 81)
Sidechain	82.0 % (487 of 594)	87.9 % (321 of 365)	71.5 % (158 of 221)	100.0 % (8 of 8)
Aromatic	36.7 % (22 of 60)	63.3 % (19 of 30)	6.9 % (2 of 29)	100.0 % (1 of 1)
Methyl	91.3 % (84 of 92)	100.0 % (46 of 46)	82.6 % (38 of 46)

1. Nucleolar protein 3

KLPAKRYRIT MKNLPEGCSW QDLKDLAREN SLETTFSSVN TRDFDGTGAL EFPSEEILVE ALERLNNIEF RGSVITVERD DNPPPIR

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	Nucleolar_protein_3	natural abundance		0.0 ~ 0.0 mM
2	Potassium phosphate			20 mM
3	potassium chloride			20 mM

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LACS Plot; CA

Referencing offset: -0.46 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.46 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.01 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 2.26 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2OSR, Strand ID: A Detail

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Release date

2008-05-05

Citation

Structure of the yeast SR protein Npl3 and Interaction with mRNA 3'-end processing signals
Deka, P., Bucheli, M., Moore, C., Buratowski, S., Varani, G.
J. Mol. Biol. (2008), 375, 136-150, PubMed 18022637 , DOI 10.1016/j.jmb.2007.09.029 , Abstract

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. Nucleolar protein 3, : 1 : 3 : 113 entities Detail

Interaction partners 1. Nucleolar protein 3, : 63 interactors Detail

Experiments performed 3 experiments Detail