BMRB: 7383

NMR Structure of RRM-1 of Yeast NPL3 Protein

Authors

Deka, P., Bucheli, M., Skrisovska, L., Allain, F.H., Moore, C., Buratowski, S., Varani, G.

Assembly

Nucleolar protein 3

Entity

1. Nucleolar protein 3 (polymer, Thiol state: not present), 74 monomers, 8346.338 Da Detail

ELSNTRLFVR PFPLDVQESE LNEIFGPFGP MKEVKILNGF AFVEFEEAES AAKAIEEVHG KSFANQPLEV VYSK

Formula weight

8346.338 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 82.0 %, Completeness (bb): 96.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	82.0 % (730 of 890)	84.5 % (392 of 464)	74.9 % (263 of 351)	100.0 % (75 of 75)
Backbone	96.1 % (417 of 434)	97.3 % (143 of 147)	94.0 % (205 of 218)	100.0 % (69 of 69)
Sidechain	71.1 % (374 of 526)	78.5 % (249 of 317)	58.6 % (119 of 203)	100.0 % (6 of 6)
Aromatic	18.5 % (17 of 92)	37.0 % (17 of 46)	0.0 % (0 of 46)
Methyl	89.7 % (70 of 78)	100.0 % (39 of 39)	79.5 % (31 of 39)

1. Nucleolar protein 3

ELSNTRLFVR PFPLDVQESE LNEIFGPFGP MKEVKILNGF AFVEFEEAES AAKAIEEVHG KSFANQPLEV VYSK

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	Nucleolar_protein_3	[U-99% 13C; U-99%15N]		1.0 (±0.05) mM
2	Potassium phosphate			20 mM
3	potassium chloride			20 mM

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LACS Plot; CA

Referencing offset: -0.25 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.25 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.05 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 2.39 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	external	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	external	direct	1.0
15N	DSS	methyl protons	0.0 ppm	external	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2OSQ, Strand ID: A Detail

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Release date

2008-05-05

Citation

Structure of the yeast SR protein Npl3 and Interaction with mRNA 3'-end processing signals
Deka, P., Bucheli, M., Moore, C., Buratowski, S., Varani, G.
J. Mol. Biol. (2008), 375, 136-150, PubMed 18022637 , DOI 10.1016/j.jmb.2007.09.029 , Abstract

Related entities 1. Nucleolar protein 3, : 1 : 6 : 392 entities Detail

Interaction partners 1. Nucleolar protein 3, : 63 interactors Detail

Experiments performed 3 experiments Detail