BMRB: 7395

Solution Structure of RWD/GI domain of Saccharomyces cerevisiae GCN2

Authors

Ogura, K., Torikai, S., Kumeta, H., Inagaki, F.

Assembly

RWD/GI domain

Entity

1. RWD/GI domain (polymer), 138 monomers, 16297.25 Da Detail

MSLSHLTLDQ YYEIQCNELE AIRSIYMDDF TDLTKRKSSW DKQPQIIFEI TLRSVDKEPV ESSITLHFAM TPMYPYTAPE IEFKNVQNVM DSQLQMLKSE FKKIHNTSRG QEIIFEITSF TQEKLDEFQN VVNTQSLE

Formula weight

16297.25 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.8 %, Completeness: 79.9 %, Completeness (bb): 76.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	79.9 % (1355 of 1695)	87.6 % (776 of 886)	68.1 % (448 of 658)	86.8 % (131 of 151)
Backbone	76.5 % (626 of 818)	90.4 % (246 of 272)	62.7 % (259 of 413)	91.0 % (121 of 133)
Sidechain	83.8 % (850 of 1014)	86.3 % (530 of 614)	81.2 % (310 of 382)	55.6 % (10 of 18)
Aromatic	65.3 % (94 of 144)	81.9 % (59 of 72)	49.3 % (35 of 71)	0.0 % (0 of 1)
Methyl	93.1 % (134 of 144)	93.1 % (67 of 72)	93.1 % (67 of 72)

1. Serine-threonine-protein kinase GCN2

MSLSHLTLDQ YYEIQCNELE AIRSIYMDDF TDLTKRKSSW DKQPQIIFEI TLRSVDKEPV ESSITLHFAM TPMYPYTAPE IEFKNVQNVM DSQLQMLKSE FKKIHNTSRG QEIIFEITSF TQEKLDEFQN VVNTQSLE

Show sample condition

Sample

Solvent system 90% H2O, 10% D2O, Pressure 1 null, Temperature 298 K, pH 6.5, Details 1mM RWD/GI domain U-15N, 13C; 20mM phosphate buffer; 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	RWD/GI domain	[U-15N; U-13C]		1 mM
2	phosphate buffer			20 mM
3	H2O			90 %
4	D2O			10 %

Hide sample codition

LACS Plot; CA

Referencing offset: 0.1 ppm, Outliers: 1 Detail

LACS Plot; CB

Referencing offset: 0.1 ppm, Outliers: 1 Detail

LACS Plot; HA

Referencing offset: 0.02 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2YZ0, Strand ID: A Detail

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Release date

2015-06-15

Citation

Solution structure of RWD/GI domain of Saccharomyces cerevisiae GCN2
Ogura, K., Torikai, S., Kumeta, H., Inagaki, F.

Related entities 1. RWD/GI domain, : 1 : 2 : 38 entities Detail

Interaction partners 1. RWD/GI domain, : 47 interactors Detail

Experiments performed 2 experiments Detail