BMRB: 7396

Solution Structure of ETO-TAFH refined in explicit solvent

Authors

Wei, Y., Liu, S., Lausen, J., Woodrell, C., Cho, S., Biris, N., Kobayashi, N., Yokoyama, S., Werner, M.H.

Assembly

ETO

Entity

1. ETO (polymer, Thiol state: all free), 107 monomers, 12122.87 Da Detail

GARQLSKLKR FLTTLQQYGN DISPEIGERV RTLVLGLVNS TLTIEEFHSK LQEATNFPLR PFVIPFLKAN LPLLQRELLH CARLAKQNPA QYLAQHEQLL LDASTTS

Formula weight

12122.87 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.1 %, Completeness: 90.7 %, Completeness (bb): 84.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	90.7 % (1175 of 1296)	97.5 % (660 of 677)	79.8 % (402 of 504)	98.3 % (113 of 115)
Backbone	84.3 % (531 of 630)	98.6 % (209 of 212)	70.0 % (222 of 317)	99.0 % (100 of 101)
Sidechain	97.1 % (747 of 769)	97.0 % (451 of 465)	97.6 % (283 of 290)	92.9 % (13 of 14)
Aromatic	83.3 % (65 of 78)	84.6 % (33 of 39)	82.1 % (32 of 39)
Methyl	100.0 % (148 of 148)	100.0 % (74 of 74)	100.0 % (74 of 74)

1. Protein ETO

GARQLSKLKR FLTTLQQYGN DISPEIGERV RTLVLGLVNS TLTIEEFHSK LQEATNFPLR PFVIPFLKAN LPLLQRELLH CARLAKQNPA QYLAQHEQLL LDASTTS

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Sample

Solvent system 95% H2O, 5% D2O, Pressure 1 null, Temperature 293 K, pH 6.8, Details 1.1 mM protein, 200 mM phosphate buffer, 95% H2O, 5% D2O

#	Name	Isotope labeling	Type	Concentration
1	ETO	natural abundance		1.1 mM
2	phosphate buffer			200 mM
3	H2O			95 %
4	D2O			5 %

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LACS Plot; CA

Referencing offset: -0.11 ppm, Outliers: 2 Detail

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Chemical shift reference

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LACS Plot; CB

Referencing offset: -0.11 ppm, Outliers: 2 Detail

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Chemical shift reference

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LACS Plot; HA

Referencing offset: 0.09 ppm, Outliers: 4 Detail

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Protein Blocks Logo

Calculated from 20 models in PDB: 2PP4, Strand ID: A Detail

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Release date

2008-06-16

Citation

A TAF4-homology domain from the corepressor ETO is a docking platform for positive and negative regulators of transcription
Wei, Y., Liu, S., Lausen, J., Woodrell, C., Cho, S., Biris, N., Kobayashi, N., Wei, Y., Yokoyama, S., Werner, M.H.
Nat. Struct. Mol. Biol. (2007), 14, 653-661, PubMed 17572682 , DOI 10.1038/nsmb1258 , Abstract

Related entities 1. ETO, : 1 : 5 : 19 entities Detail

Interaction partners 1. ETO, : 2 interactors Detail

Experiments performed 2 experiments Detail