BMRB: 7402

Spatial Structure and Membrane Permeabilization for Latarcin-1, a Spider Antimicrobial Peptide

Authors

Dubovskii, P.V., Volynsky, P.E., Polyansky, A.A., Chupin, V.V., Efremov, R.G., Arseniev, A.S.

Assembly

Latarcin-1

Entity

1. Latarcin-1 (polymer, Thiol state: not present), 26 monomers, 3201.942 Da Detail

SMWSGMWRRK LKKLRNALKK KLKGEK

Formula weight

3201.942 Da

Source organism

Lachesana tarabaevi

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 70.7 %, Completeness (bb): 92.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	70.7 % (140 of 198)	70.7 % (140 of 198)
Backbone	92.6 % (50 of 54)	92.6 % (50 of 54)
Sidechain	62.5 % (90 of 144)	62.5 % (90 of 144)
Aromatic	100.0 % (12 of 12)	100.0 % (12 of 12)
Methyl	100.0 % (9 of 9)	100.0 % (9 of 9)

1. Latarcin-1

SMWSGMWRRK LKKLRNALKK KLKGEK

Show sample condition

Sample #1

Solvent system 90% H2O, 10% D2O, Pressure 1 atm, Temperature 318 K, pH 7.1, Details 2 mM Ltc1, 120 mM perdeuterated SDS, pH 7.1, salt-free, 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Ltc1	natural abundance		2 mM
2	perdeuterated SDS			120 mM
3	H2O			90 %
4	D2O			10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 318 K, pH 7.1, Details 2 mM Ltc1, 120 mM perdeuterated SDS, pH 7.1, salt-free, 100% D2O

#	Name	Isotope labeling	Type	Concentration
5	Ltc1	natural abundance		2 mM
6	perdeuterated SDS			120 mM
7	D2O			100 %

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Calculated from 20 models in PDB: 2PCO, Strand ID: A Detail

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Release date

2008-07-07

Citation

Three-dimensional structure/hydrophobicity of latarcins specifies their mode of membrane activity
Dubovskii, P.V., Volynsky, P.E., Polyansky, A.A., Karpunin, D.V., Chupin, V.V., Efremov, R.G., Arseniev, A.S.
Biochemistry (2008), 47, 3525-3533, PubMed 18293934 , DOI 10.1021/bi702203w , Abstract

Related entities 1. Latarcin-1, : 1 : 1 entities Detail

Experiments performed 4 experiments Detail