BMRB: 7419

SOLUTION STRUCTURE OF AMINO TERMINAL DOMAIN OF HUMAN DNA POLYMERASE EPSILON SUBUNIT B REVEALS ITS HOMOLOGY TO C-DOMAINS OF AAA PROTEINS.

Authors

Nuutinen, T., Fredriksson, K., Tossavainen, H., Pospiech, H., Pirila, P., Permi, P., Annila, A., Syvaoja, J.E.

Assembly

entity 1

Entity

1. entity 1 (polymer, Thiol state: not present), 99 monomers, 11069.37 Da Detail

MGSSHHHHHH SQDPNSSSAR LQVDMAPERL RSRALSAFKL RGLLLRGEAI KYLTEALQSI SELELEDKLE KIINAVEKQP LSSNMIERSV VEAAVQESS

Formula weight

11069.37 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.9 %, Completeness: 89.1 %, Completeness (bb): 89.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	89.1 % (1012 of 1136)	89.6 % (533 of 595)	89.0 % (389 of 437)	86.5 % (90 of 104)
Backbone	89.5 % (526 of 588)	90.4 % (179 of 198)	88.8 % (261 of 294)	89.6 % (86 of 96)
Sidechain	89.0 % (573 of 644)	89.2 % (354 of 397)	90.0 % (215 of 239)	50.0 % (4 of 8)
Aromatic	42.9 % (18 of 42)	42.9 % (9 of 21)	42.9 % (9 of 21)
Methyl	100.0 % (112 of 112)	100.0 % (56 of 56)	100.0 % (56 of 56)

1. AAA protein

MGSSHHHHHH SQDPNSSSAR LQVDMAPERL RSRALSAFKL RGLLLRGEAI KYLTEALQSI SELELEDKLE KIINAVEKQP LSSNMIERSV VEAAVQESS

Show sample condition

Sample

Pressure 1.0 atm, Temperature 298.0 K, pH 6.62, Details 93%WATER/7%D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	natural abundance		0.0 ~ 0.0 mM

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LACS Plot; CA

Referencing offset: -0.11 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

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LACS Plot; CB

Referencing offset: -0.11 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

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LACS Plot; HA

Referencing offset: -0.01 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

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LACS Plot; CO

Referencing offset: -0.04 ppm, Outliers: 5 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

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Protein Blocks Logo

Calculated from 20 models in PDB: 2V6Z, Strand ID: M Detail

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Release date

2008-08-21

Citation

The solution structure of the amino-terminal domain of human DNA polymerase epsilon subunit B is homologous to C-domains of AAA+ proteins
Nuutinen, T., Tossavainen, H., Fredriksson, K., Pirila, P., Permi, P., Pospiech, H., Syvaoja, J.E.
Nucleic Acids Res. (2008), 36, 5102-5110, PubMed 18676977 , DOI 10.1093/nar/gkn497 , Abstract

Related entities 1. entity 1, : 1 entities Detail

Experiments performed 8 experiments Detail