BMRB: 7421

STRUCTURE OF CCP MODULE 7 OF COMPLEMENT FACTOR H- THE AMD NOT AT RISK VARIENT (402Y)

Authors

Herbert, A.P., Deakin, J.A., Schmidt, C.Q., Blaum, B.S., Egan, C., Ferreira, V., Pangburn, M.K., Lyon, M., Uhrin, D., Barlow, P.N.

Assembly

COMPLEMENT FACTOR H

Entity

1. COMPLEMENT FACTOR H (polymer), 65 monomers, 7328.329 Da Detail

EAAGLRKCYF PYLENGYNQN YGRKFVQGKS IDVACHPGYA LPKAQTTVTC MENGWSPTPR CIRVK

Formula weight

7328.329 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

ARIA 2.0

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 95.5 %, Completeness (bb): 95.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.5 % (728 of 762)	95.7 % (383 of 400)	95.2 % (280 of 294)	95.6 % (65 of 68)
Backbone	95.3 % (362 of 380)	95.4 % (125 of 131)	95.2 % (180 of 189)	95.0 % (57 of 60)
Sidechain	96.1 % (424 of 441)	95.9 % (258 of 269)	96.3 % (158 of 164)	100.0 % (8 of 8)
Aromatic	94.7 % (72 of 76)	94.7 % (36 of 38)	94.6 % (35 of 37)	100.0 % (1 of 1)
Methyl	96.3 % (52 of 54)	100.0 % (27 of 27)	92.6 % (25 of 27)

1. COMPLEMENT FACTOR H

EAAGLRKCYF PYLENGYNQN YGRKFVQGKS IDVACHPGYA LPKAQTTVTC MENGWSPTPR CIRVK

Show sample condition

Sample

Pressure 1.0 atm, Temperature 298.0 K, pH 5.2, Details 10% WATER/90% D2O

#	Name	Isotope labeling	Type	Concentration
1	COMPLEMENT FACTOR H	natural abundance		0.0 ~ 0.0 mM

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LACS Plot; CA

Referencing offset: -0.18 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

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LACS Plot; CB

Referencing offset: -0.18 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

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LACS Plot; HA

Referencing offset: 0.0 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

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LACS Plot; CO

Referencing offset: 0.55 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	na	na	0.0 ppm	null	null	0.0
1H	na	na	0.0 ppm	null	null	0.0
15N	na	na	0.0 ppm	null	null	0.0

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Protein Blocks Logo

Calculated from 20 models in PDB: 2JGX, Strand ID: A Detail

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Release date

2008-08-07

Citation

Structure shows that a glycosaminoglycan and protein recognition site in factor H is perturbed by age-related macular degeneration-linked single nucleotide polymorphism
Herbert, A.P., Deakin, J.A., Schmidt, C.Q., Blaum, B.S., Egan, C., Ferreira, V., Pangburn, M.K., Lyon, M., Uhrin, D., Barlow, P.N.
J. Biol. Chem. (2007), 282, 18960-18968, PubMed 17360715 , DOI 10.1074/jbc.M609636200 , Abstract

Related entities 1. COMPLEMENT FACTOR H, : 1 : 3 : 257 entities Detail

Interaction partners 1. COMPLEMENT FACTOR H, : 17 interactors Detail

Experiments performed 10 experiments Detail