BMRBj - BMREntryMaster

Found 1 Document (1.638 seconds)

BMRB: 9500

1VDJ

Structure of actin-interacting domain of troponin

Authors

Murakami, K., Yumoto, F., Ohki, S., Yasunaga, T., Tanokura, M., Wakabayashi, T.

Assembly

troponin complex

Entity

1. TnI (polymer, Thiol state: not present), 52 monomers, 6125.877 Da Detail

KVNMDLRANL KQVKKEDTEK EKDLRDVGDW RKNIEEKSGM EGRKKMFEAG ES

2. CA (non-polymer), na Da

Formula weight

6125.877 Da

Source organism

Gallus gallus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.2 %, Completeness: 30.8 %, Completeness (bb): 61.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	30.8 % (195 of 634)	14.6 % (50 of 343)	41.5 % (97 of 234)	84.2 % (48 of 57)
Backbone	61.5 % (192 of 312)	46.3 % (50 of 108)	61.8 % (94 of 152)	92.3 % (48 of 52)
Sidechain	12.7 % (47 of 370)	0.0 % (0 of 235)	36.2 % (47 of 130)	0.0 % (0 of 5)
Aromatic	0.0 % (0 of 22)	0.0 % (0 of 11)	0.0 % (0 of 10)	0.0 % (0 of 1)
Methyl	5.9 % (2 of 34)	0.0 % (0 of 17)	11.8 % (2 of 17)

1. TnI

KVNMDLRANL KQVKKEDTEK EKDLRDVGDW RKNIEEKSGM EGRKKMFEAG ES

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 7, Details The troponin ternary complex high Ca2+

#	Name	Isotope labeling	Concentration
1	Troponin I	[U-13C; U-15N]	0.8 ~ 1.2 mM
2	Troponin C	natural abundance	0.8 ~ 1.2 mM
3	Troponin T2	natural abundance	0.8 ~ 1.2 mM
4	KCl		240 mM
5	NaHCO3		0.3 mM
6	MgCl2		3 mM
7	CaCl2		0.1 mM
8	H2O		90 %
9	D2O		10 %

Sample #2

Solvent system 100% D2O, Temperature 298 K, pH 7, Details The troponin ternary complex high Ca2+

#	Name	Isotope labeling	Concentration
10	Troponin I	[U-13C; U-15N]	0.8 ~ 1.2 mM
11	Troponin C	natural abundance	0.8 ~ 1.2 mM
12	Troponin T2	natural abundance	0.8 ~ 1.2 mM
13	KCl		240 mM
14	NaHCO3		0.3 mM
15	MgCl2		3 mM
16	CaCl2		0.1 mM
17	D2O		100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	na	indirect	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.24 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	na	indirect	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.24 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1VDJ, Strand ID: A Detail

Release date

2009-05-20

Citation

Structural basis for Ca2+-regulated muscle relaxation at interaction sites of troponin with actin and tropomyosin
Murakami, K., Yumoto, F., Ohki, S., Yasunaga, T., Tanokura, M., Wakabayashi, T.
J. Mol. Biol. (2005), 352, 178-201, PubMed 16061251 , DOI 10.1016/j.jmb.2005.06.067 , Abstract

Related entities 1. TnI, : 1 : 3 : 42 entities Detail

Interaction partners 1. TnI, : 2 interactors Detail

More details for 2 interactors identified by 1 citations

Experiments performed 12 experiments Detail

1 HNCACB

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 7, Details The troponin ternary complex high Ca2+

#	Name	Isotope labeling	Concentration
1	Troponin I	[U-13C; U-15N]	0.8 ~ 1.2 mM
2	Troponin C	natural abundance	0.8 ~ 1.2 mM
3	Troponin T2	natural abundance	0.8 ~ 1.2 mM
4	KCl		240 mM
5	NaHCO3		0.3 mM
6	MgCl2		3 mM
7	CaCl2		0.1 mM
8	H2O		90 %
9	D2O		10 %

2 CBCA(CO)NH

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 7, Details The troponin ternary complex high Ca2+

#	Name	Isotope labeling	Concentration
1	Troponin I	[U-13C; U-15N]	0.8 ~ 1.2 mM
2	Troponin C	natural abundance	0.8 ~ 1.2 mM
3	Troponin T2	natural abundance	0.8 ~ 1.2 mM
4	KCl		240 mM
5	NaHCO3		0.3 mM
6	MgCl2		3 mM
7	CaCl2		0.1 mM
8	H2O		90 %
9	D2O		10 %

3 HNCA

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 7, Details The troponin ternary complex high Ca2+

#	Name	Isotope labeling	Concentration
1	Troponin I	[U-13C; U-15N]	0.8 ~ 1.2 mM
2	Troponin C	natural abundance	0.8 ~ 1.2 mM
3	Troponin T2	natural abundance	0.8 ~ 1.2 mM
4	KCl		240 mM
5	NaHCO3		0.3 mM
6	MgCl2		3 mM
7	CaCl2		0.1 mM
8	H2O		90 %
9	D2O		10 %

4 HN(CO)CA

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 7, Details The troponin ternary complex high Ca2+

#	Name	Isotope labeling	Concentration
1	Troponin I	[U-13C; U-15N]	0.8 ~ 1.2 mM
2	Troponin C	natural abundance	0.8 ~ 1.2 mM
3	Troponin T2	natural abundance	0.8 ~ 1.2 mM
4	KCl		240 mM
5	NaHCO3		0.3 mM
6	MgCl2		3 mM
7	CaCl2		0.1 mM
8	H2O		90 %
9	D2O		10 %

5 HNCO

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 7, Details The troponin ternary complex high Ca2+

#	Name	Isotope labeling	Concentration
1	Troponin I	[U-13C; U-15N]	0.8 ~ 1.2 mM
2	Troponin C	natural abundance	0.8 ~ 1.2 mM
3	Troponin T2	natural abundance	0.8 ~ 1.2 mM
4	KCl		240 mM
5	NaHCO3		0.3 mM
6	MgCl2		3 mM
7	CaCl2		0.1 mM
8	H2O		90 %
9	D2O		10 %

6 H(CCO)NH

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 7, Details The troponin ternary complex high Ca2+

#	Name	Isotope labeling	Concentration
1	Troponin I	[U-13C; U-15N]	0.8 ~ 1.2 mM
2	Troponin C	natural abundance	0.8 ~ 1.2 mM
3	Troponin T2	natural abundance	0.8 ~ 1.2 mM
4	KCl		240 mM
5	NaHCO3		0.3 mM
6	MgCl2		3 mM
7	CaCl2		0.1 mM
8	H2O		90 %
9	D2O		10 %

7 C(CO)NH

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 7, Details The troponin ternary complex high Ca2+

#	Name	Isotope labeling	Concentration
1	Troponin I	[U-13C; U-15N]	0.8 ~ 1.2 mM
2	Troponin C	natural abundance	0.8 ~ 1.2 mM
3	Troponin T2	natural abundance	0.8 ~ 1.2 mM
4	KCl		240 mM
5	NaHCO3		0.3 mM
6	MgCl2		3 mM
7	CaCl2		0.1 mM
8	H2O		90 %
9	D2O		10 %

8 HCCH-TOCSY

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 7, Details The troponin ternary complex high Ca2+

#	Name	Isotope labeling	Concentration
1	Troponin I	[U-13C; U-15N]	0.8 ~ 1.2 mM
2	Troponin C	natural abundance	0.8 ~ 1.2 mM
3	Troponin T2	natural abundance	0.8 ~ 1.2 mM
4	KCl		240 mM
5	NaHCO3		0.3 mM
6	MgCl2		3 mM
7	CaCl2		0.1 mM
8	H2O		90 %
9	D2O		10 %

9 2D 13C-NOESY

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 7, Details The troponin ternary complex high Ca2+

#	Name	Isotope labeling	Concentration
1	Troponin I	[U-13C; U-15N]	0.8 ~ 1.2 mM
2	Troponin C	natural abundance	0.8 ~ 1.2 mM
3	Troponin T2	natural abundance	0.8 ~ 1.2 mM
4	KCl		240 mM
5	NaHCO3		0.3 mM
6	MgCl2		3 mM
7	CaCl2		0.1 mM
8	H2O		90 %
9	D2O		10 %

10 2D aromatic 13C-edited NOESY

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Temperature 298 K, pH 7, Details The troponin ternary complex high Ca2+

#	Name	Isotope labeling	Concentration
10	Troponin I	[U-13C; U-15N]	0.8 ~ 1.2 mM
11	Troponin C	natural abundance	0.8 ~ 1.2 mM
12	Troponin T2	natural abundance	0.8 ~ 1.2 mM
13	KCl		240 mM
14	NaHCO3		0.3 mM
15	MgCl2		3 mM
16	CaCl2		0.1 mM
17	D2O		100 %

11 3D 13C-edited NOESY

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 7, Details The troponin ternary complex high Ca2+

#	Name	Isotope labeling	Concentration
1	Troponin I	[U-13C; U-15N]	0.8 ~ 1.2 mM
2	Troponin C	natural abundance	0.8 ~ 1.2 mM
3	Troponin T2	natural abundance	0.8 ~ 1.2 mM
4	KCl		240 mM
5	NaHCO3		0.3 mM
6	MgCl2		3 mM
7	CaCl2		0.1 mM
8	H2O		90 %
9	D2O		10 %

12 3D 15N-edited NOESY

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 7, Details The troponin ternary complex high Ca2+

#	Name	Isotope labeling	Concentration
1	Troponin I	[U-13C; U-15N]	0.8 ~ 1.2 mM
2	Troponin C	natural abundance	0.8 ~ 1.2 mM
3	Troponin T2	natural abundance	0.8 ~ 1.2 mM
4	KCl		240 mM
5	NaHCO3		0.3 mM
6	MgCl2		3 mM
7	CaCl2		0.1 mM
8	H2O		90 %
9	D2O		10 %

nullKeywords actin, tropomyosin, troponin

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Found 1 Document (1.638 seconds)

BMRB: 9500

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Related entities 1. TnI, : 1 : 3 : 42 entities Detail

Interaction partners 1. TnI, : 2 interactors Detail

Experiments performed 12 experiments Detail

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