BMRBj - BMREntryMaster

Found 1 Document (1.706 seconds)

BMRB: 9500

1VDJ

Structure of actin-interacting domain of troponin

Authors

Murakami, K., Yumoto, F., Ohki, S., Yasunaga, T., Tanokura, M., Wakabayashi, T.

Assembly

troponin complex

Entity

1. TnI (polymer, Thiol state: not present), 52 monomers, 6125.877 Da Detail

KVNMDLRANL KQVKKEDTEK EKDLRDVGDW RKNIEEKSGM EGRKKMFEAG ES

2. CA (non-polymer), na Da

Formula weight

6125.877 Da

Source organism

Gallus gallus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.2 %, Completeness: 30.8 %, Completeness (bb): 61.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	30.8 % (195 of 634)	14.6 % (50 of 343)	41.5 % (97 of 234)	84.2 % (48 of 57)
Backbone	61.5 % (192 of 312)	46.3 % (50 of 108)	61.8 % (94 of 152)	92.3 % (48 of 52)
Sidechain	12.7 % (47 of 370)	0.0 % (0 of 235)	36.2 % (47 of 130)	0.0 % (0 of 5)
Aromatic	0.0 % (0 of 22)	0.0 % (0 of 11)	0.0 % (0 of 10)	0.0 % (0 of 1)
Methyl	5.9 % (2 of 34)	0.0 % (0 of 17)	11.8 % (2 of 17)

1. TnI

KVNMDLRANL KQVKKEDTEK EKDLRDVGDW RKNIEEKSGM EGRKKMFEAG ES

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 7, Details The troponin ternary complex high Ca2+

#	Name	Isotope labeling	Concentration
1	Troponin I	[U-13C; U-15N]	0.8 ~ 1.2 mM
2	Troponin C	natural abundance	0.8 ~ 1.2 mM
3	Troponin T2	natural abundance	0.8 ~ 1.2 mM
4	KCl		240 mM
5	NaHCO3		0.3 mM
6	MgCl2		3 mM
7	CaCl2		0.1 mM
8	H2O		90 %
9	D2O		10 %

Sample #2

Solvent system 100% D2O, Temperature 298 K, pH 7, Details The troponin ternary complex high Ca2+

#	Name	Isotope labeling	Concentration
10	Troponin I	[U-13C; U-15N]	0.8 ~ 1.2 mM
11	Troponin C	natural abundance	0.8 ~ 1.2 mM
12	Troponin T2	natural abundance	0.8 ~ 1.2 mM
13	KCl		240 mM
14	NaHCO3		0.3 mM
15	MgCl2		3 mM
16	CaCl2		0.1 mM
17	D2O		100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	na	indirect	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.24 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	na	indirect	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.24 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1VDJ, Strand ID: A Detail

Release date

2009-05-20

Citation

Structural basis for Ca2+-regulated muscle relaxation at interaction sites of troponin with actin and tropomyosin
Murakami, K., Yumoto, F., Ohki, S., Yasunaga, T., Tanokura, M., Wakabayashi, T.
J. Mol. Biol. (2005), 352, 178-201, PubMed 16061251 , DOI 10.1016/j.jmb.2005.06.067 , Abstract

Related entities 1. TnI, : 1 : 3 : 42 entities Detail

Interaction partners 1. TnI, : 2 interactors Detail

More details for 2 interactors identified by 1 citations

Experiments performed 12 experiments Detail

1 HNCACB

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 7, Details The troponin ternary complex high Ca2+

#	Name	Isotope labeling	Concentration
1	Troponin I	[U-13C; U-15N]	0.8 ~ 1.2 mM
2	Troponin C	natural abundance	0.8 ~ 1.2 mM
3	Troponin T2	natural abundance	0.8 ~ 1.2 mM
4	KCl		240 mM
5	NaHCO3		0.3 mM
6	MgCl2		3 mM
7	CaCl2		0.1 mM
8	H2O		90 %
9	D2O		10 %

2 CBCA(CO)NH

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 7, Details The troponin ternary complex high Ca2+

#	Name	Isotope labeling	Concentration
1	Troponin I	[U-13C; U-15N]	0.8 ~ 1.2 mM
2	Troponin C	natural abundance	0.8 ~ 1.2 mM
3	Troponin T2	natural abundance	0.8 ~ 1.2 mM
4	KCl		240 mM
5	NaHCO3		0.3 mM
6	MgCl2		3 mM
7	CaCl2		0.1 mM
8	H2O		90 %
9	D2O		10 %

3 HNCA

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 7, Details The troponin ternary complex high Ca2+

#	Name	Isotope labeling	Concentration
1	Troponin I	[U-13C; U-15N]	0.8 ~ 1.2 mM
2	Troponin C	natural abundance	0.8 ~ 1.2 mM
3	Troponin T2	natural abundance	0.8 ~ 1.2 mM
4	KCl		240 mM
5	NaHCO3		0.3 mM
6	MgCl2		3 mM
7	CaCl2		0.1 mM
8	H2O		90 %
9	D2O		10 %

4 HN(CO)CA

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 7, Details The troponin ternary complex high Ca2+

#	Name	Isotope labeling	Concentration
1	Troponin I	[U-13C; U-15N]	0.8 ~ 1.2 mM
2	Troponin C	natural abundance	0.8 ~ 1.2 mM
3	Troponin T2	natural abundance	0.8 ~ 1.2 mM
4	KCl		240 mM
5	NaHCO3		0.3 mM
6	MgCl2		3 mM
7	CaCl2		0.1 mM
8	H2O		90 %
9	D2O		10 %

5 HNCO

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 7, Details The troponin ternary complex high Ca2+

#	Name	Isotope labeling	Concentration
1	Troponin I	[U-13C; U-15N]	0.8 ~ 1.2 mM
2	Troponin C	natural abundance	0.8 ~ 1.2 mM
3	Troponin T2	natural abundance	0.8 ~ 1.2 mM
4	KCl		240 mM
5	NaHCO3		0.3 mM
6	MgCl2		3 mM
7	CaCl2		0.1 mM
8	H2O		90 %
9	D2O		10 %

6 H(CCO)NH

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 7, Details The troponin ternary complex high Ca2+

#	Name	Isotope labeling	Concentration
1	Troponin I	[U-13C; U-15N]	0.8 ~ 1.2 mM
2	Troponin C	natural abundance	0.8 ~ 1.2 mM
3	Troponin T2	natural abundance	0.8 ~ 1.2 mM
4	KCl		240 mM
5	NaHCO3		0.3 mM
6	MgCl2		3 mM
7	CaCl2		0.1 mM
8	H2O		90 %
9	D2O		10 %

7 C(CO)NH

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 7, Details The troponin ternary complex high Ca2+

#	Name	Isotope labeling	Concentration
1	Troponin I	[U-13C; U-15N]	0.8 ~ 1.2 mM
2	Troponin C	natural abundance	0.8 ~ 1.2 mM
3	Troponin T2	natural abundance	0.8 ~ 1.2 mM
4	KCl		240 mM
5	NaHCO3		0.3 mM
6	MgCl2		3 mM
7	CaCl2		0.1 mM
8	H2O		90 %
9	D2O		10 %

8 HCCH-TOCSY

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 7, Details The troponin ternary complex high Ca2+

#	Name	Isotope labeling	Concentration
1	Troponin I	[U-13C; U-15N]	0.8 ~ 1.2 mM
2	Troponin C	natural abundance	0.8 ~ 1.2 mM
3	Troponin T2	natural abundance	0.8 ~ 1.2 mM
4	KCl		240 mM
5	NaHCO3		0.3 mM
6	MgCl2		3 mM
7	CaCl2		0.1 mM
8	H2O		90 %
9	D2O		10 %

9 2D 13C-NOESY

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 7, Details The troponin ternary complex high Ca2+

#	Name	Isotope labeling	Concentration
1	Troponin I	[U-13C; U-15N]	0.8 ~ 1.2 mM
2	Troponin C	natural abundance	0.8 ~ 1.2 mM
3	Troponin T2	natural abundance	0.8 ~ 1.2 mM
4	KCl		240 mM
5	NaHCO3		0.3 mM
6	MgCl2		3 mM
7	CaCl2		0.1 mM
8	H2O		90 %
9	D2O		10 %

10 2D aromatic 13C-edited NOESY

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Temperature 298 K, pH 7, Details The troponin ternary complex high Ca2+

#	Name	Isotope labeling	Concentration
10	Troponin I	[U-13C; U-15N]	0.8 ~ 1.2 mM
11	Troponin C	natural abundance	0.8 ~ 1.2 mM
12	Troponin T2	natural abundance	0.8 ~ 1.2 mM
13	KCl		240 mM
14	NaHCO3		0.3 mM
15	MgCl2		3 mM
16	CaCl2		0.1 mM
17	D2O		100 %

11 3D 13C-edited NOESY

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 7, Details The troponin ternary complex high Ca2+

#	Name	Isotope labeling	Concentration
1	Troponin I	[U-13C; U-15N]	0.8 ~ 1.2 mM
2	Troponin C	natural abundance	0.8 ~ 1.2 mM
3	Troponin T2	natural abundance	0.8 ~ 1.2 mM
4	KCl		240 mM
5	NaHCO3		0.3 mM
6	MgCl2		3 mM
7	CaCl2		0.1 mM
8	H2O		90 %
9	D2O		10 %

12 3D 15N-edited NOESY

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 7, Details The troponin ternary complex high Ca2+

#	Name	Isotope labeling	Concentration
1	Troponin I	[U-13C; U-15N]	0.8 ~ 1.2 mM
2	Troponin C	natural abundance	0.8 ~ 1.2 mM
3	Troponin T2	natural abundance	0.8 ~ 1.2 mM
4	KCl		240 mM
5	NaHCO3		0.3 mM
6	MgCl2		3 mM
7	CaCl2		0.1 mM
8	H2O		90 %
9	D2O		10 %

nullKeywords actin, tropomyosin, troponin

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (1.706 seconds)

BMRB: 9500

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Related entities 1. TnI, : 1 : 3 : 42 entities Detail

Interaction partners 1. TnI, : 2 interactors Detail

Experiments performed 12 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample