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2FYH

1H, 13C and 15N resonance assignments of the 2'-5' RNA ligase-like protein from Pyrococcus furiosus

Authors

Okada, K., Matsuda, T., Sakamoto, T., Muto, Y., Yokoyama, S., Kanai, A., Kawai, G.

Assembly

2'-5' RNA ligase-like protein

Entity

1. 2'-5' RNA ligase-like protein (polymer, Thiol state: not present), 190 monomers, 21818.02 Da Detail

MRAFIAIDVS ESVRDALVRA QDYIGSKEAK IKFVERENFH ITLKFLGEIT EEQAEEIKKI LEKIAKKYKK HEVNVRGIGV FPNPNYVRVI WAGVENDEII KKIAKEIDDE LAKLGFKKEG NFVAHITLGR VKFVKDKLGL AMKLKELANE DFGSFIVEAI ELKKSTLTPK GPIYETLARF ELSEHHHHHH

Formula weight

21818.02 Da

Source organism

Pyrococcus furiosus DSM 3638

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.9 %, Completeness (bb): 98.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	97.9 % (2285 of 2334)	97.9 % (1195 of 1221)	97.9 % (898 of 917)	98.0 % (192 of 196)
Backbone	98.9 % (1120 of 1132)	99.0 % (383 of 387)	99.1 % (554 of 559)	98.4 % (183 of 186)
Sidechain	97.3 % (1344 of 1381)	97.4 % (812 of 834)	97.4 % (523 of 537)	90.0 % (9 of 10)
Aromatic	85.3 % (162 of 190)	86.3 % (82 of 95)	85.1 % (80 of 94)	0.0 % (0 of 1)
Methyl	100.0 % (234 of 234)	100.0 % (117 of 117)	100.0 % (117 of 117)

1. 2'-5' RNA ligase-like protein

Show sample condition

Sample

Temperature 313 (±0.1) K, pH 4.7 (±0.1)

#	Name	Isotope labeling	Concentration
1	2'-5' RNA ligase-like protein	[U-13C; U-15N]	1.1 (±0.1) mM
2	hydrochloric acid		1.0 (±0.1) mM
3	sodium chloride		200 (±0.1) mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.17 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.17 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 5 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.13 ppm, Outliers: 1 Detail

Release date

2006-04-26

Citation

1H, 13C and 15N resonance assignments of the 2'-5' RNA ligase-like protein from Pyrococcus furiosus
Okada, K., Matsuda, T., Sakamoto, T., Muto, Y., Yokoyama, S., Kanai, A., Kawai, G.
J. Biomol. NMR (2006), 36 Suppl 1, 16-16, PubMed 16456701 , DOI 10.1007/s10858-005-5581-8 , Abstract

Related entities 1. 2'-5' RNA ligase-like protein, : 1 : 1 : 18 entities Detail

Experiments performed 13 experiments Detail

1 1H-15N HSQC