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Solution structure of the first mbt domain from human KIAA1798 protein
Authors
Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.
Assembly
KIAA1798 protein
Entity
1. KIAA1798 protein (polymer, Thiol state: all free), 127 monomers, 14023.54 Da Detail

GSSGSSGPYN KNGFKVGMKL EGVDPEHQSV YCVLTVAEVC GYRIKLHFDG YSDCYDFWVN ADALDIHPVG WCEKTGHKLH PPKGYKEEEF NWQTYLKTCK AQAAPKSLFE NQNITVIPSG FSGPSSG


Formula weight
14023.54 Da
Source organism
Homo sapiens
Exptl. method
NMR
Refine. method
torsion angle dynamics
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 96.9 %, Completeness: 93.3 %, Completeness (bb): 92.4 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All93.3 % (1363 of 1461)91.7 % (695 of 758)95.8 % (547 of 571)91.7 % (121 of 132)
Backbone92.4 % (689 of 746)89.6 % (233 of 260)94.3 % (346 of 367)92.4 % (110 of 119)
Sidechain94.6 % (783 of 828)92.8 % (462 of 498)97.8 % (310 of 317)84.6 % (11 of 13)
Aromatic98.8 % (170 of 172)97.7 % (84 of 86)100.0 % (83 of 83)100.0 % (3 of 3)
Methyl100.0 % (102 of 102)100.0 % (51 of 51)100.0 % (51 of 51)

1. mbt domain

GSSGSSGPYN KNGFKVGMKL EGVDPEHQSV YCVLTVAEVC GYRIKLHFDG YSDCYDFWVN ADALDIHPVG WCEKTGHKLH PPKGYKEEEF NWQTYLKTCK AQAAPKSLFE NQNITVIPSG FSGPSSG

Sample

Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)


#NameIsotope labelingTypeConcentration
1mbt domain[U-13C; U-15N]1.03 mM
2d-Tris-HClbuffer20 mM
3NaClsalt100 mM
4d-DTTsalt1 mM
5NaN30.02 %
6H2Osolvent90 %
7D2Osolvent10 %

LACS Plot; CA
Referencing offset: -0.06 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.06 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: -0.01 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.17 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 1WJS, Strand ID: A Detail


Release date
2008-08-12
Citation
Solution NMR structure of the DNA-binding domain from Scml2 (sex comb on midleg-like 2)
Tomizawa, T., Kigawa, T., Koshiba, S., Inoue, M., Yokoyama, S.
J. Biol. Chem. (2014), 289, 15739-15749, PubMed 24727478 , DOI 10.1074/jbc.M113.524009 ,
Related entities 1. KIAA1798 protein, : 1 : 5 : 89 entities Detail
Interaction partners 1. KIAA1798 protein, : 40 interactors Detail
Experiments performed 2 experiments Detail
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