Solution Structure of the Tudor Domain from Mouse Hypothetical Protein Homologous to Histone Acetyltransferase
GSSGSSGEPE VTVEIGETYL CRRPDSTWHS AEVIQSRVND QEGREEFYVH YVGFNRRLDE WVDKNRLALT KTVKDAVQKN SEKYLSELAE QPERKITRNQ KRKHDEINHV QKTYAEMDPT TAALEKESGP SSG
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 84.4 % (1297 of 1536) | 82.4 % (666 of 808) | 87.7 % (514 of 586) | 82.4 % (117 of 142) |
Backbone | 85.4 % (673 of 788) | 83.3 % (224 of 269) | 87.2 % (341 of 391) | 84.4 % (108 of 128) |
Sidechain | 82.8 % (723 of 873) | 80.7 % (435 of 539) | 87.2 % (279 of 320) | 64.3 % (9 of 14) |
Aromatic | 92.0 % (92 of 100) | 92.0 % (46 of 50) | 91.7 % (44 of 48) | 100.0 % (2 of 2) |
Methyl | 93.1 % (108 of 116) | 93.1 % (54 of 58) | 93.1 % (54 of 58) |
1. Tudor domain
GSSGSSGEPE VTVEIGETYL CRRPDSTWHS AEVIQSRVND QEGREEFYVH YVGFNRRLDE WVDKNRLALT KTVKDAVQKN SEKYLSELAE QPERKITRNQ KRKHDEINHV QKTYAEMDPT TAALEKESGP SSGPressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Tudor domain | [U-13C, U-15N] | 1.06 mM | |
2 | d-Tris-HCl | buffer | 20 mM | |
3 | NaCl | salt | 100 mM | |
4 | d-DTT | salt | 1 mM | |
5 | NaN3 | 0.02 % | ||
6 | H2O | solvent | 90 % | |
7 | D2O | solvent | 10 % |