BMRB: 21011

LPS bound structure of Cysteine deleted TachyplesinI

Authors

Bhattacharjya, S., Saravanan, R.

Assembly

PROTEIN

Entity

1. PROTEIN (polymer, Thiol state: not present), 13 monomers, 1856.187 Da Detail

KWFRVYRGIY RRR

Formula weight

1856.187 Da

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.3 %, Completeness: 69.5 %, Completeness (bb): 88.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	69.5 % (73 of 105)	69.5 % (73 of 105)
Backbone	88.9 % (24 of 27)	88.9 % (24 of 27)
Sidechain	62.8 % (49 of 78)	62.8 % (49 of 78)
Aromatic	42.1 % (8 of 19)	42.1 % (8 of 19)
Methyl	100.0 % (4 of 4)	100.0 % (4 of 4)

1. CDT

KWFRVYRGIY RRR

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Temperature 298 K, pH 4.8, Details 500micromolar of CDT in LPS micelles

#	Name	Isotope labeling	Type	Concentration
1	CDT	natural abundance	protein	500 uM
2	LPS	natural abundance	micelle	10 uM
3	H2O		solvent	90 %
4	D2O		solvent	10 %

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Release date

2011-06-22

Citation

Structure, activity and interactions of the cysteine deleted analog of tachyplesin-1 with lipopolysaccharide micelle: Mechanistic insights into outer-membrane permeabilization and endotoxin neutralization
Saravanan, R., Mohanram, H., Joshii, M., Domadiai, P., Torresi, J., Ruedl, C., Bhattacharjya, S.
Biochim. Biophys. Acta (2012), 1818, 1613-1624, PubMed 22464970 , DOI 10.1016/j.bbamem.2012.03.015 , Abstract

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. PROTEIN, : 1 entities Detail

Experiments performed 2 experiments Detail

Chemical shift validation 3 contents Detail