1H and 15N assignment of reduced Hydrogenobacter thermophilus cytochrome c552
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | thioether | sing | 1:CYS10:SG | 2:HEC1:CAB |
2 | thioether | sing | 1:CYS13:SG | 2:HEC1:CAC |
3 | metal coordination | sing | 1:HIS14:NE | 2:HEC1:FE |
4 | metal coordination | sing | 1:MET59:SD | 2:HEC1:FE |
Polymer type: polypeptide(L)
Total | 1H | 15N | |
---|---|---|---|
All | 29.1 % (166 of 570) | 18.8 % (91 of 485) | 88.2 % (75 of 85) |
Backbone | 63.7 % (153 of 240) | 47.6 % (78 of 164) | 98.7 % (75 of 76) |
Sidechain | 3.9 % (13 of 330) | 4.0 % (13 of 321) | 0.0 % (0 of 9) |
Aromatic | 0.0 % (0 of 28) | 0.0 % (0 of 26) | 0.0 % (0 of 2) |
Methyl | 4.9 % (2 of 41) | 4.9 % (2 of 41) |
1. cytochrome c552
NEQLAKQKGC MACHDLKAKK VGPAYADVAK KYAGRKDAVD YLAGKIKKGG SGVWGSVPMP PQNVTDAEAK QLAQWILSIKTemperature 296 (±2) K, pH 6.0 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | cytochrome c552 | [U-15N] | 1 mM | |
2 | potassium phosphate | 20 mM | ||
3 | sodium hydrosulfite | 100 mM |