Solution structure of the second WW domain from the human membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1. MAGI-1
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 92.3 % (631 of 684) | 89.4 % (322 of 360) | 95.8 % (252 of 263) | 93.4 % (57 of 61) |
Backbone | 92.0 % (324 of 352) | 87.8 % (108 of 123) | 94.8 % (164 of 173) | 92.9 % (52 of 56) |
Sidechain | 93.0 % (358 of 385) | 90.3 % (214 of 237) | 97.2 % (139 of 143) | 100.0 % (5 of 5) |
Aromatic | 100.0 % (48 of 48) | 100.0 % (24 of 24) | 100.0 % (23 of 23) | 100.0 % (1 of 1) |
Methyl | 98.0 % (49 of 50) | 96.0 % (24 of 25) | 100.0 % (25 of 25) |
1. WW domain
GSSGSSGLDS ELELPAGWEK IEDPVYGIYY VDHINRKTQY ENPVLEAKRK KQLESGPSSGSolvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | WW domain | [U-13C; U-15N] | protein | 1.0 mM |
2 | d-Tris-HCl | buffer | 20 mM | |
3 | NaCl | salt | 100 mM | |
4 | d-DTT | salt | 1 mM | |
5 | NaN3 | salt | 0.02 mM | |
6 | H2O | solvent | 90 % | |
7 | D2O | solvent | 10 % |