Solution structures of the WHEP-TRS domain of human methionyl-tRNA synthetase
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 85.5 % (761 of 890) | 84.6 % (401 of 474) | 86.8 % (290 of 334) | 85.4 % (70 of 82) |
Backbone | 83.4 % (387 of 464) | 82.0 % (132 of 161) | 84.3 % (193 of 229) | 83.8 % (62 of 74) |
Sidechain | 87.7 % (436 of 497) | 85.9 % (269 of 313) | 90.3 % (159 of 176) | 100.0 % (8 of 8) |
Methyl | 100.0 % (78 of 78) | 100.0 % (39 of 39) | 100.0 % (39 of 39) |
1. WHEP-TRS domain
GSSGSSGTTA KPQQIQALMD EVTKQGNIVR ELKAQKADKN EVAAEVAKLL DLKKQLAVAE GKPPEAPKGK KKKSGPSSGSolvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | WHWP-TRS domain | [U-13C; U-15N] | protein | 1 mM |
2 | d-Tris HCl | buffer | 20 mM | |
3 | NaCl | salt | 100 mM | |
4 | d-DTT | salt | 1 mM | |
5 | NaN3 | salt | 0.02 % | |
6 | H2O | solvent | 90 % | |
7 | D2O | solvent | 10 % |