Liquid crystal solution structure of the kissing complex formed by the apical loop of the HIV TAR RNA and a high affinity RNA aptamer optimized by SELEX.
Polymer type: polyribonucleotide
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 80.4 % (410 of 510) | 83.3 % (235 of 282) | 82.5 % (175 of 212) | 0.0 % (0 of 16) |
Suger, PO4 | 87.8 % (309 of 352) | 87.0 % (167 of 192) | 88.7 % (142 of 160) | |
Nucleobase | 63.9 % (101 of 158) | 75.6 % (68 of 90) | 63.5 % (33 of 52) | 0.0 % (0 of 16) |
Aromatic | 74.3 % (101 of 136) | 100.0 % (68 of 68) | 63.5 % (33 of 52) | 0.0 % (0 of 16) |
1. TAR
GAGCCCUGGG AGGCUC2. TAR*GA
GCUGGUCCCA GACAGCSolvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.6
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
37 | TAR | natural abundance | 1.8 mM | |
38 | TAR*GA | [U-98% 13C; U-98% 15N] | 0.9 mM | |
39 | sodium phosphate | natural abundance | 10 mM | |
40 | sodium chloride | natural abundance | 50 mM | |
41 | EDTA | natural abundance | 10 uM | |
42 | sodium azide | natural abundance | 0.4 g/L |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.6
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
43 | TAR | [U-98% 13C; U-98% 15N] | 0.9 mM | |
44 | TAR*GA | natural abundance | 1.8 mM | |
45 | sodium phosphate | natural abundance | 10 mM | |
46 | sodium chloride | natural abundance | 50 mM | |
47 | EDTA | natural abundance | 10 uM | |
48 | sodium azide | natural abundance | 0.4 g/L |