Solution Structure of the N-terminal SAP Domain of SUMO E3 Ligases from Saccharomyces cerevisiae
GSHMINLEDY WEDETPGPDR EPTNELRNEV EETITLMELL KVSELKDICR SVSFPVSGRK AVLQDLIRNF LQNALVVGKS DPYRVQAVKF LIERIRKNEP LPVYKDLWNA LRKG
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 94.9 % (1312 of 1382) | 95.7 % (697 of 728) | 93.3 % (499 of 535) | 97.5 % (116 of 119) |
Backbone | 98.1 % (657 of 670) | 98.7 % (223 of 226) | 97.6 % (329 of 337) | 98.1 % (105 of 107) |
Sidechain | 92.9 % (763 of 821) | 94.4 % (474 of 502) | 90.6 % (278 of 307) | 91.7 % (11 of 12) |
Aromatic | 72.0 % (59 of 82) | 85.4 % (35 of 41) | 59.0 % (23 of 39) | 50.0 % (1 of 2) |
Methyl | 97.1 % (132 of 136) | 100.0 % (68 of 68) | 94.1 % (64 of 68) |
1. Siz1 1-111
GSHMINLEDY WEDETPGPDR EPTNELRNEV EETITLMELL KVSELKDICR SVSFPVSGRK AVLQDLIRNF LQNALVVGKS DPYRVQAVKF LIERIRKNEP LPVYKDLWNA LRKGSolvent system 92% H2O/8% D2O, Pressure 1 atm, Temperature 283 K, pH 6.1
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Siz1 1-111 | [U-15N] | 0.55 mM | |
2 | potassium phosphate | natural abundance | 20 mM | |
3 | sodium chloride | natural abundance | 300 mM | |
4 | DTT | natural abundance | 3 mM | |
5 | H2O | natural abundance | 92 % | |
6 | D2O | 8 % |
Solvent system 92% H2O/8% D2O, Pressure 1 atm, Temperature 283 K, pH 6.1
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | Siz1 1-111 | [U-13C; U-15N] | 0.6 mM | |
8 | potassium phosphate | natural abundance | 20 mM | |
9 | sodium chloride | natural abundance | 300 mM | |
10 | DTT | natural abundance | 3 mM | |
11 | H2O | natural abundance | 92 % | |
12 | D2O | 8 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 283 K, pH 6.1
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
13 | Siz1 1-111 | [U-13C; U-15N] | 0.69 mM | |
14 | potassium phosphate | natural abundance | 20 mM | |
15 | sodium chloride | natural abundance | 300 mM | |
16 | DTT | natural abundance | 3 mM | |
17 | D2O | 100 % |