BMRB: 11047

RecO-bound ssDNA

Authors

Shibata, T., Ito, Y.

Assembly

5'-D(*DTP*DAP*DCP*DG)-3' 10 structures

Entity

1. DNA (5'-D(*DTP*DAP*DCP*DG)-3') (polymer, Thiol state: not present), 4 monomers, 1253.802 × 10 Da Detail

TACG

2. ttRecO (polymer, Thiol state: not present), 229 monomers, 24801.69 Da Detail

MAPAYTGKVE RYRLEEGIVV GRKPLPQGDL LLRLVTPRGS LEAVVRKGQR PTGRTGRLSL FHHVRFQLYA KGEGLPTLTQ AELLGRLHGL EAPRRFLLAA FLAELAYRLA SPEAAPRIYP LLVSGLRGIA KHEDPLLPLV WAGWRVAKAG GIGPNLEGEG LRLKRGRLGE EGVYLGREGV EALKATLRLP GAQALPHLEG APLNRLFLAL KAHAEEALGP LRSAEAIGV

Total weight

37339.71 Da

Max. entity weight

24801.69 Da

Source organism

Thermus thermophilus HB8

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 3.9 %, Completeness: 3.5 %, Completeness (bb): 7.2 % Detail

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Polymer type: polydeoxyribonucleotide polypeptide(L)

	Total	1H
All	3.5 % (50 of 1420)	3.5 % (50 of 1420)
Backbone	7.2 % (36 of 498)	7.2 % (36 of 498)
Sidechain	1.5 % (14 of 922)	1.5 % (14 of 922)
Aromatic	7.4 % (6 of 81)	7.4 % (6 of 81)
Methyl	1.3 % (2 of 158)	1.3 % (2 of 158)

1. DNA (5'-D(*DTP*DAP*DCP*DG)-3')

TACG

2. ttRecO

MAPAYTGKVE RYRLEEGIVV GRKPLPQGDL LLRLVTPRGS LEAVVRKGQR PTGRTGRLSL FHHVRFQLYA KGEGLPTLTQ AELLGRLHGL EAPRRFLLAA FLAELAYRLA SPEAAPRIYP LLVSGLRGIA KHEDPLLPLV WAGWRVAKAG GIGPNLEGEG LRLKRGRLGE EGVYLGREGV EALKATLRLP GAQALPHLEG APLNRLFLAL KAHAEEALGP LRSAEAIGV

Show sample condition

Sample

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.1

#	Name	Isotope labeling	Type	Concentration
1	DNA (5'-D(*DTP*DAP*DCP*DG)-3')	natural abundance	DNA	0.5 mM
2	ttRecO	natural abundance	protein	0.05 mM
3	MgCl2	natural abundance	salt	5 mM
4	NaCl	natural abundance	salt	150 mM
5	D2O		solvent	100 %

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Release date

2009-10-14

Citation

A non-canonical DNA structure enables homologous recombination in various genetic systems
Masuda, T., Ito, Y., Terada, T., Shibata, T., Mikawa, T.
J. Biol. Chem. (2009), 284, 30230-30239, PubMed 19729448 , DOI 10.1074/jbc.M109.043810 , Abstract

Related entities 2. ttRecO, : 2 entities Detail

Experiments performed 5 experiments Detail

Chemical shift validation 4 contents Detail