Solution structure of mouse lipocalin-type prostaglandin D synthase possessing a intrinsic disulfide bond.
GSQGHDTVQP NFQQDKFLGR WYSAGLASNS SWFREKKAVL YMAKTVVAPS TEGGLNLTST FLRKNQCETK IMVLQPAGAP GHYTYSSPHS GSIHSVSVVE ANYDEYALLF SRGTKGPGQD FRMATLYSRT QTLKDELKEK FTTFSKAQGL TEEDIVFLPQ PDKCIQE
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 1:CYS67:SG | 1:CYS164:SG |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 72.2 % (1403 of 1942) | 73.8 % (745 of 1009) | 64.7 % (489 of 756) | 95.5 % (169 of 177) |
Backbone | 82.8 % (816 of 986) | 97.6 % (331 of 339) | 67.6 % (330 of 488) | 97.5 % (155 of 159) |
Sidechain | 66.5 % (738 of 1110) | 61.8 % (414 of 670) | 73.5 % (310 of 422) | 77.8 % (14 of 18) |
Aromatic | 23.1 % (43 of 186) | 28.0 % (26 of 93) | 16.5 % (15 of 91) | 100.0 % (2 of 2) |
Methyl | 84.2 % (133 of 158) | 84.8 % (67 of 79) | 83.5 % (66 of 79) |
1. lipocalin-type prostaglandin D synthase
GSQGHDTVQP NFQQDKFLGR WYSAGLASNS SWFREKKAVL YMAKTVVAPS TEGGLNLTST FLRKNQCETK IMVLQPAGAP GHYTYSSPHS GSIHSVSVVE ANYDEYALLF SRGTKGPGQD FRMATLYSRT QTLKDELKEK FTTFSKAQGL TEEDIVFLPQ PDKCIQESolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 4
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Prostaglandin-H2 D-isomerase | [U-13C; U-15N] | 0.5 mM | |
2 | acetic acid | [U-2H] | 20 mM | |
3 | H2O | 90 % | ||
4 | D2O | 10 % |
Solvent system 99.9% D2O, Pressure 1 atm, Temperature 298 K, pH 4
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | Prostaglandin-H2 D-isomerase | [U-13C; U-15N] | 0.5 mM | |
6 | acetic acid | [U-2H] | 20 mM | |
7 | D2O | 99.9 % |