BMRBj - BMREntryMaster

Found 1 Document (0.601 seconds)

BMRB: 11075

2RPA

1H, 13C, and 15N Chemical Shift Assignments for N-terminal domain of kp60

Authors

Iwaya, N., Kuwahara, Y., Tenno, T., Tochio, H., Shirakawa, M., Hiroaki, H.

Assembly

kp60

Entity

1. kp60 (polymer, Thiol state: not present), 78 monomers, 9245.548 Da Detail

GSDHMTMSLQ MIVENVKLAR EYALLGNYDS AMVYYQGVLD QMNKYLYSVK DTHLRQKWQQ VWQEINVEAK QVKDIMKT

Formula weight

9245.548 Da

Source organism

Exptl. method

Refine. method

distance geometry, rigid body restrained molecular dynamics, dihedral angle molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.7 %, Completeness: 88.1 %, Completeness (bb): 83.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	88.1 % (846 of 960)	93.4 % (468 of 501)	79.6 % (292 of 367)	93.5 % (86 of 92)
Backbone	83.3 % (390 of 468)	95.0 % (151 of 159)	70.1 % (162 of 231)	98.7 % (77 of 78)
Sidechain	93.5 % (530 of 567)	92.7 % (317 of 342)	96.7 % (204 of 211)	64.3 % (9 of 14)
Aromatic	97.5 % (78 of 80)	97.5 % (39 of 40)	97.4 % (37 of 38)	100.0 % (2 of 2)
Methyl	100.0 % (86 of 86)	100.0 % (43 of 43)	100.0 % (43 of 43)

1. N-terminal domain of kp60

GSDHMTMSLQ MIVENVKLAR EYALLGNYDS AMVYYQGVLD QMNKYLYSVK DTHLRQKWQQ VWQEINVEAK QVKDIMKT

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	N-terminal domain of kp60	[U-13C; U-15N]		0.5 mM
2	sodium phosphate	natural abundance		20 mM
3	EDTA	natural abundance		1 mM
4	H2O	natural abundance		95 %
5	D2O	natural abundance		5 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
6	N-terminal domain of kp60	[U-13C; U-15N]		0.7 mM
7	sodium phosphate	natural abundance		20 mM
8	sodium chloride	natural abundance		50 mM
9	EDTA	natural abundance		1 mM
10	H2O	natural abundance		95 %
11	D2O	natural abundance		5 %

Protein Blocks Logo

Calculated from 20 models in PDB: 2RPA, Strand ID: A Detail

Release date

2010-04-06

Citation

A common substrate recognition mode conserved between katanin p60 and VPS4 governs microtubule severing and membrane skeleton reorganization
Iwaya, N., Kuwahara, Y., Fujiwara, Y., Goda, N., Tenno, T., Akiyama, K., Mase, S., Tochio, H., Ikegami, T., Shirakawa, M., Hiroaki, H.
J. Biol. Chem. (2010), 285, 16822-16829, PubMed 20339000 , DOI 10.1074/jbc.M110.108365 , Abstract

Related entities 1. kp60, : 1 : 6 : 38 entities Detail

Interaction partners 1. kp60, : 2 interactors Detail

More details for 2 interactors identified by 1 citations

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	N-terminal domain of kp60	[U-13C; U-15N]		0.5 mM
2	sodium phosphate	natural abundance		20 mM
3	EDTA	natural abundance		1 mM
4	H2O	natural abundance		95 %
5	D2O	natural abundance		5 %

2 2D 1H-13C HSQC

Instrument

Bruker DMX - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
6	N-terminal domain of kp60	[U-13C; U-15N]		0.7 mM
7	sodium phosphate	natural abundance		20 mM
8	sodium chloride	natural abundance		50 mM
9	EDTA	natural abundance		1 mM
10	H2O	natural abundance		95 %
11	D2O	natural abundance		5 %

3 3D CBCA(CO)NH

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	N-terminal domain of kp60	[U-13C; U-15N]		0.5 mM
2	sodium phosphate	natural abundance		20 mM
3	EDTA	natural abundance		1 mM
4	H2O	natural abundance		95 %
5	D2O	natural abundance		5 %

4 3D C(CO)NH

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
6	N-terminal domain of kp60	[U-13C; U-15N]		0.7 mM
7	sodium phosphate	natural abundance		20 mM
8	sodium chloride	natural abundance		50 mM
9	EDTA	natural abundance		1 mM
10	H2O	natural abundance		95 %
11	D2O	natural abundance		5 %

5 3D HNCO

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	N-terminal domain of kp60	[U-13C; U-15N]		0.5 mM
2	sodium phosphate	natural abundance		20 mM
3	EDTA	natural abundance		1 mM
4	H2O	natural abundance		95 %
5	D2O	natural abundance		5 %

6 3D HNCACB

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	N-terminal domain of kp60	[U-13C; U-15N]		0.5 mM
2	sodium phosphate	natural abundance		20 mM
3	EDTA	natural abundance		1 mM
4	H2O	natural abundance		95 %
5	D2O	natural abundance		5 %

7 3D H(CCO)NH

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
6	N-terminal domain of kp60	[U-13C; U-15N]		0.7 mM
7	sodium phosphate	natural abundance		20 mM
8	sodium chloride	natural abundance		50 mM
9	EDTA	natural abundance		1 mM
10	H2O	natural abundance		95 %
11	D2O	natural abundance		5 %

8 3D HCCH-TOCSY

Instrument

Bruker DMX - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
6	N-terminal domain of kp60	[U-13C; U-15N]		0.7 mM
7	sodium phosphate	natural abundance		20 mM
8	sodium chloride	natural abundance		50 mM
9	EDTA	natural abundance		1 mM
10	H2O	natural abundance		95 %
11	D2O	natural abundance		5 %

9 3D 1H-15N NOESY

Instrument

Varian INOVA - 900 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
6	N-terminal domain of kp60	[U-13C; U-15N]		0.7 mM
7	sodium phosphate	natural abundance		20 mM
8	sodium chloride	natural abundance		50 mM
9	EDTA	natural abundance		1 mM
10	H2O	natural abundance		95 %
11	D2O	natural abundance		5 %

10 3D 1H-13C NOESY

Instrument

Bruker DMX - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
6	N-terminal domain of kp60	[U-13C; U-15N]		0.7 mM
7	sodium phosphate	natural abundance		20 mM
8	sodium chloride	natural abundance		50 mM
9	EDTA	natural abundance		1 mM
10	H2O	natural abundance		95 %
11	D2O	natural abundance		5 %

11 3D HNCA

Instrument

Bruker DMX - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	N-terminal domain of kp60	[U-13C; U-15N]		0.5 mM
2	sodium phosphate	natural abundance		20 mM
3	EDTA	natural abundance		1 mM
4	H2O	natural abundance		95 %
5	D2O	natural abundance		5 %

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr11075_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr11075_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	938		98.7 (chain: 1, length: 78)
1	Chemical shift references	chemical_shift_reference_1	Warning	3		No information

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 78, Sequence coverage: 98.7%
- Total number of assignments
  - ¹H chemical shifts: 574
  - ¹³C chemical shifts: 279
  - ¹⁵N chemical shifts: 85
- Completeness of assignments
  - Entity_assemble_ID: 1
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Keywords kp60