BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	96.1 % (689 of 717)	94.6 % (350 of 370)	98.9 % (279 of 282)	92.3 % (60 of 65)
Backbone	98.3 % (356 of 362)	98.4 % (123 of 125)	98.9 % (176 of 178)	96.6 % (57 of 59)
Sidechain	94.6 % (389 of 411)	92.7 % (227 of 245)	99.4 % (159 of 160)	50.0 % (3 of 6)
Aromatic	97.1 % (68 of 70)	97.1 % (34 of 35)	97.0 % (32 of 33)	100.0 % (2 of 2)
Methyl	100.0 % (72 of 72)	100.0 % (36 of 36)	100.0 % (36 of 36)

1. DDEF1 SH3

VRRVKTIYDC QADNDDELTF IEGEVIIVTG EEDQEWWIGH IEGQPERKGV FPVSFVHILS D

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
1	DDEF1 SH3	[U-15N]	protein	0.7 mM
2	sodium phosphate	natural abundance	buffer	20 mM
3	sodium chloride	natural abundance	salt	50 mM
4	sodium azide	natural abundance	salt	0.02 %
5	H2O		solvent	90 %
6	D2O		solvent	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
7	DDEF1 SH3	[U-13C; U-15N]	protein	0.7 mM
8	sodium phosphate	natural abundance	buffer	20 mM
9	sodium chloride	natural abundance	salt	50 mM
10	sodium azide	natural abundance	salt	0.02 %
11	H2O		solvent	90 %
12	D2O		solvent	10 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
13	DDEF1 SH3	[U-13C; U-15N]	protein	0.7 mM
14	sodium phosphate	natural abundance	buffer	20 mM
15	sodium chloride	natural abundance	salt	50 mM
16	sodium azide	natural abundance	salt	0.02 %
17	D2O		solvent	100 %

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
18	DDEF1 SH3	[U-15% 13C; U-15N]	protein	0.7 mM
19	sodium phosphate	natural abundance	buffer	20 mM
20	sodium chloride	natural abundance	salt	50 mM
21	sodium azide	natural abundance	salt	0.02 %
22	H2O		solvent	90 %
23	D2O		solvent	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	internal	indirect	0.2514495
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	water	protons	4.773 ppm	internal	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	internal	indirect	0.2514495
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	water	protons	4.773 ppm	internal	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	internal	indirect	0.2514495
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	water	protons	4.773 ppm	internal	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	internal	indirect	0.2514495
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	water	protons	4.773 ppm	internal	indirect	0.1013291

Referencing offset: 0.34 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2RQT, Strand ID: A Detail

Release date

2011-05-18

Citation

Structural basis of the recognition of the SAMP motif of adenomatous polyposis coli by the Src-homology 3 domain
Kaieda, S., Matsui, C., Mimori-Kiyosue, Y., Ikegami, T.
Biochemistry (2010), 49, 5143-5153, PubMed 20509626 , DOI 10.1021/bi100563z , Abstract

Entries sharing articles BMRB: 1, Swiss-Prot: 2 entries Detail

  BMRB: 11082 released on 2011-05-18
    Title Solution structure of the complex between the DDEF1 SH3 domain and the APC SAMP1 motif
  Swiss-Prot: Q9ULH1 released on 2003-09-26
    Title ASAP1_HUMAN Entity Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1
  Swiss-Prot: P25054 released on 1992-05-01
    Title APC_HUMAN Entity Adenomatous polyposis coli protein

Related entities 1. DDEF1 SH3, : 1 : 9 : 1 : 394 entities Detail

Interaction partners 1. DDEF1 SH3, : 11 interactors Detail

More details for 11 interactors identified by 7 citations

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
1	DDEF1 SH3	[U-15N]	protein	0.7 mM
2	sodium phosphate	natural abundance	buffer	20 mM
3	sodium chloride	natural abundance	salt	50 mM
4	sodium azide	natural abundance	salt	0.02 %
5	H2O		solvent	90 %
6	D2O		solvent	10 %

2 3D HNCACB

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
7	DDEF1 SH3	[U-13C; U-15N]	protein	0.7 mM
8	sodium phosphate	natural abundance	buffer	20 mM
9	sodium chloride	natural abundance	salt	50 mM
10	sodium azide	natural abundance	salt	0.02 %
11	H2O		solvent	90 %
12	D2O		solvent	10 %

3 3D CBCA(CO)NH

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
7	DDEF1 SH3	[U-13C; U-15N]	protein	0.7 mM
8	sodium phosphate	natural abundance	buffer	20 mM
9	sodium chloride	natural abundance	salt	50 mM
10	sodium azide	natural abundance	salt	0.02 %
11	H2O		solvent	90 %
12	D2O		solvent	10 %

4 3D HNCO

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
7	DDEF1 SH3	[U-13C; U-15N]	protein	0.7 mM
8	sodium phosphate	natural abundance	buffer	20 mM
9	sodium chloride	natural abundance	salt	50 mM
10	sodium azide	natural abundance	salt	0.02 %
11	H2O		solvent	90 %
12	D2O		solvent	10 %

5 3D HN(CA)CO

Instrument

Bruker DRX - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
7	DDEF1 SH3	[U-13C; U-15N]	protein	0.7 mM
8	sodium phosphate	natural abundance	buffer	20 mM
9	sodium chloride	natural abundance	salt	50 mM
10	sodium azide	natural abundance	salt	0.02 %
11	H2O		solvent	90 %
12	D2O		solvent	10 %

6 3D H(CCO)NH

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
7	DDEF1 SH3	[U-13C; U-15N]	protein	0.7 mM
8	sodium phosphate	natural abundance	buffer	20 mM
9	sodium chloride	natural abundance	salt	50 mM
10	sodium azide	natural abundance	salt	0.02 %
11	H2O		solvent	90 %
12	D2O		solvent	10 %

7 3D HCCH-TOCSY

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
13	DDEF1 SH3	[U-13C; U-15N]	protein	0.7 mM
14	sodium phosphate	natural abundance	buffer	20 mM
15	sodium chloride	natural abundance	salt	50 mM
16	sodium azide	natural abundance	salt	0.02 %
17	D2O		solvent	100 %

8 3D 15N-edited NOESY

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
1	DDEF1 SH3	[U-15N]	protein	0.7 mM
2	sodium phosphate	natural abundance	buffer	20 mM
3	sodium chloride	natural abundance	salt	50 mM
4	sodium azide	natural abundance	salt	0.02 %
5	H2O		solvent	90 %
6	D2O		solvent	10 %

9 3D 13C-edited NOESY

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
13	DDEF1 SH3	[U-13C; U-15N]	protein	0.7 mM
14	sodium phosphate	natural abundance	buffer	20 mM
15	sodium chloride	natural abundance	salt	50 mM
16	sodium azide	natural abundance	salt	0.02 %
17	D2O		solvent	100 %

10 2D 1H-13C HSQC

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
18	DDEF1 SH3	[U-15% 13C; U-15N]	protein	0.7 mM
19	sodium phosphate	natural abundance	buffer	20 mM
20	sodium chloride	natural abundance	salt	50 mM
21	sodium azide	natural abundance	salt	0.02 %
22	H2O		solvent	90 %
23	D2O		solvent	10 %

11 2D 1H-13C CT-HSQC

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
18	DDEF1 SH3	[U-15% 13C; U-15N]	protein	0.7 mM
19	sodium phosphate	natural abundance	buffer	20 mM
20	sodium chloride	natural abundance	salt	50 mM
21	sodium azide	natural abundance	salt	0.02 %
22	H2O		solvent	90 %
23	D2O		solvent	10 %

nullKeywords GAP, Protein, SH3 domain

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Found 1 Document (2.271 seconds)

BMRB: 11081

Sample #1

Sample #2

Sample #3

Sample #4

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles BMRB: 1, Swiss-Prot: 2 entries Detail

Related entities 1. DDEF1 SH3, : 1 : 9 : 1 : 394 entities Detail

Interaction partners 1. DDEF1 SH3, : 11 interactors Detail

Experiments performed 11 experiments Detail

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