BMRB: 11083

Solution structure of the Leucine Rich Repeat of human Acidic leucine-rich nuclear phosphoprotein 32 family member B

Authors

Tochio, N., Koshiba, S., Watanabe, S., Harada, T., Umehara, T., Tanaka, A., Kigawa, T., Yokoyama, S.

Assembly

Acidic leucine-rich nuclear phosphoprotein 32 family member B

Entity

1. Acidic leucine-rich nuclear phosphoprotein 32 family member B (polymer, Thiol state: all free), 168 monomers, 18818.35 Da Detail

GSSGSSGMDM KRRIHLELRN RTPAAVRELV LDNCKSNDGK IEGLTAEFVN LEFLSLINVG LISVSNLPKL PKLKKLELSE NRIFGGLDML AEKLPNLTHL NLSGNKLKDI STLEPLKKLE CLKSLDLFNC EVTNLNDYRE SVFKLLPQLT YLDGYDREDQ EAPDSDAE

Formula weight

18818.35 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.4 %, Completeness: 96.3 %, Completeness (bb): 97.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	96.3 % (1902 of 1976)	94.7 % (981 of 1036)	97.8 % (747 of 764)	98.9 % (174 of 176)
Backbone	97.8 % (972 of 994)	96.5 % (327 of 339)	98.4 % (486 of 494)	98.8 % (159 of 161)
Sidechain	95.3 % (1086 of 1140)	93.8 % (654 of 697)	97.4 % (417 of 428)	100.0 % (15 of 15)
Aromatic	90.2 % (74 of 82)	95.1 % (39 of 41)	85.4 % (35 of 41)
Methyl	99.0 % (202 of 204)	100.0 % (102 of 102)	98.0 % (100 of 102)

1. LRR 1 domain

GSSGSSGMDM KRRIHLELRN RTPAAVRELV LDNCKSNDGK IEGLTAEFVN LEFLSLINVG LISVSNLPKL PKLKKLELSE NRIFGGLDML AEKLPNLTHL NLSGNKLKDI STLEPLKKLE CLKSLDLFNC EVTNLNDYRE SVFKLLPQLT YLDGYDREDQ EAPDSDAE

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Sample

Solvent system 90% H2O, 10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 1.3mM sample [U-15N, 13C], 20mM d-Tris-HCl, 100mM NaCl, 1mM d-DTT, 0.02% NaN3, 90% H2O, 10% D2O

#	Name	Isotope labeling	Type	Concentration
1	LRR_1 domain	[U-13C; U-15N]	protein	1.3 mM
2	TRIS	[U-2H]	buffer	20 mM
3	sodium chloride	natural abundance	salt	100 mM
4	DTT	[U-2H]	salt	1 mM
5	sodium azide	natural abundance	salt	0.02 %
6	H2O		solvent	90 %
7	D2O		solvent	10 %

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LACS Plot; CA

Referencing offset: -0.05 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.05 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.05 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.4 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	null	indirect	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2ELL, Strand ID: A Detail

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Release date

2010-05-26

Citation

Solution structure of the Leucine Rich Repeat of human Acidic leucine-rich nuclear phosphoprotein 32 family member B
Tochio, N., Koshiba, S., Watanabe, S., Harada, T., Umehara, T., Tanaka, A., Kigawa, T., Yokoyama, S.

Related entities 1. Acidic leucine-rich nuclear phosphoprotein 32 family member B, : 1 : 1 : 2 : 112 entities Detail

Interaction partners 1. Acidic leucine-rich nuclear phosphoprotein 32 family member B, : 35 interactors Detail

Experiments performed 2 experiments Detail