The solution structure of the fifth fibronectin type III domain of human Neogenin
GSSGSSGPMM PPVGVQASIL SHDTIRITWA DNSLPKHQKI TDSRYYTVRW KTNIPANTKY KNANATTLSY LVTGLKPNTL YEFSVMVTKG RRSSTWSMTA HGTTFELSGP SSG
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 93.3 % (1189 of 1275) | 92.2 % (606 of 657) | 95.0 % (476 of 501) | 91.5 % (107 of 117) |
Backbone | 92.0 % (611 of 664) | 90.4 % (206 of 228) | 93.6 % (309 of 330) | 90.6 % (96 of 106) |
Sidechain | 94.7 % (677 of 715) | 93.2 % (400 of 429) | 96.7 % (266 of 275) | 100.0 % (11 of 11) |
Aromatic | 96.3 % (104 of 108) | 96.3 % (52 of 54) | 96.1 % (49 of 51) | 100.0 % (3 of 3) |
Methyl | 95.6 % (109 of 114) | 94.7 % (54 of 57) | 96.5 % (55 of 57) |
1. fn3 domain
GSSGSSGPMM PPVGVQASIL SHDTIRITWA DNSLPKHQKI TDSRYYTVRW KTNIPANTKY KNANATTLSY LVTGLKPNTL YEFSVMVTKG RRSSTWSMTA HGTTFELSGP SSGSolvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 296 (±0.1) K, pH 7.0 (±0.05), Details 1.3mM FN3 domain {U-15N,13C;} 20mM {d-Tris HCl;} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | FN3 domain | [U-13C; U-15N] | protein | 1.3 mM |
2 | d-Tris HCl | natural abundance | buffer | 20 mM |
3 | NaCl | natural abundance | salt | 100 mM |
4 | d-DTT | natural abundance | salt | 1 mM |
5 | NaN3 | natural abundance | salt | 0.02 % |
6 | H2O | solvent | 90 % | |
7 | D2O | solvent | 10 % |