Solution structure of calponin homology domain of IQGAP1
GPLGSLTAEE MDERRRQNVA YEYLCHLEEA KRWMEACLGE DLPPTTELEE GLRNGVYLAK LGNFFSPKVV SLKKIYDREQ TRYKATGLHF RHTDNVIQWL NAMDEIGLPK IFYPETTDIY DRKNMPRCIY CIHALSLYLF KLGLAPQIQD LYGKVDFTEE EINNMKTELE KYGIQMPAFS KIGGILANEL
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 84.6 % (1941 of 2295) | 89.2 % (1071 of 1201) | 76.8 % (688 of 896) | 91.9 % (182 of 198) |
Backbone | 83.5 % (937 of 1122) | 96.4 % (370 of 384) | 71.6 % (399 of 557) | 92.8 % (168 of 181) |
Sidechain | 87.5 % (1181 of 1350) | 85.8 % (701 of 817) | 90.3 % (466 of 516) | 82.4 % (14 of 17) |
Aromatic | 53.5 % (106 of 198) | 53.5 % (53 of 99) | 52.6 % (51 of 97) | 100.0 % (2 of 2) |
Methyl | 100.0 % (206 of 206) | 100.0 % (103 of 103) | 100.0 % (103 of 103) |
1. CH domain
GPLGSLTAEE MDERRRQNVA YEYLCHLEEA KRWMEACLGE DLPPTTELEE GLRNGVYLAK LGNFFSPKVV SLKKIYDREQ TRYKATGLHF RHTDNVIQWL NAMDEIGLPK IFYPETTDIY DRKNMPRCIY CIHALSLYLF KLGLAPQIQD LYGKVDFTEE EINNMKTELE KYGIQMPAFS KIGGILANELSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.4
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | CH domain | [U-99% 13C; U-99% 15N] | 0.90 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | sodium chloride | natural abundance | 150 mM | |
4 | DTT | natural abundance | 1 mM | |
5 | sodium azide | natural abundance | 1 mM | |
6 | H2O | 90 % | ||
7 | D2O | 10 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 7.4
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
8 | CH domain | [U-99% 13C; U-99% 15N] | 0.90 mM | |
9 | sodium phosphate | natural abundance | 20 mM | |
10 | sodium chloride | natural abundance | 150 mM | |
11 | DTT | natural abundance | 1 mM | |
12 | sodium azide | natural abundance | 1 mM | |
13 | D2O | 100 % |