The solution structure of the C-terminal region of Zinc finger FYVE domain-containing protein 21
GSSGSSGAEF YDKQLKVLLS GATFLVTFGN SEKPETMTCR LSNNQRYLFL DGDSHYEIEI VHISTVQILT EGFPPGGGNA RATGMFLQYT VPGTEGVTQL KLTVVEDVTV GRRQAVAWLV AMHKAAKLLY ESRDQSGPSS G
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 95.4 % (1506 of 1579) | 95.2 % (772 of 811) | 96.0 % (595 of 620) | 93.9 % (139 of 148) |
Backbone | 93.8 % (784 of 836) | 92.5 % (271 of 293) | 94.8 % (386 of 407) | 93.4 % (127 of 136) |
Sidechain | 97.2 % (844 of 868) | 96.7 % (501 of 518) | 97.9 % (331 of 338) | 100.0 % (12 of 12) |
Aromatic | 93.5 % (116 of 124) | 93.5 % (58 of 62) | 93.4 % (57 of 61) | 100.0 % (1 of 1) |
Methyl | 100.0 % (162 of 162) | 100.0 % (81 of 81) | 100.0 % (81 of 81) |
1. C-terminal region of ZFYVE21
GSSGSSGAEF YDKQLKVLLS GATFLVTFGN SEKPETMTCR LSNNQRYLFL DGDSHYEIEI VHISTVQILT EGFPPGGGNA RATGMFLQYT VPGTEGVTQL KLTVVEDVTV GRRQAVAWLV AMHKAAKLLY ESRDQSGPSS GSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 296 K, pH 7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | C-terminal region of ZFYVE21 | [U-13C; U-15N] | protein | 1.18 mM |
2 | TRIS | [U-2H] | buffer | 20 mM |
3 | sodium chloride | natural abundance | salt | 100 mM |
4 | DTT | [U-2H] | salt | 1 mM |
5 | sodium azide | natural abundance | salt | 0.02 % |
6 | D2O | [U-2H] | solvent | 10 % |
7 | H2O | natural abundance | solvent | 90 % |