Assigned chemical shifts of the zf-CW domain with H3 trimethyl K4 peptide
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | na | sing | 1:CYS21:SG | 2:ZN1:ZN |
2 | na | sing | 1:CYS26:SG | 2:ZN1:ZN |
3 | na | sing | 1:CYS47:SG | 2:ZN1:ZN |
4 | na | sing | 1:CYS58:SG | 2:ZN1:ZN |
Polymer type: polypeptide(L)
Total | 1H | 15N | |
---|---|---|---|
All | 19.5 % (120 of 614) | 12.2 % (63 of 516) | 58.2 % (57 of 98) |
Backbone | 43.2 % (114 of 264) | 32.0 % (58 of 181) | 67.5 % (56 of 83) |
Sidechain | 1.4 % (5 of 350) | 1.5 % (5 of 335) | 0.0 % (0 of 15) |
Aromatic | 0.0 % (0 of 42) | 0.0 % (0 of 38) | 0.0 % (0 of 4) |
Methyl | 6.1 % (2 of 33) | 6.1 % (2 of 33) |
1. zf-CW domain
GSSGSSGEIS GFGQCLVWVQ CSFPNCGKWR RLCGNIDPSV LPDNWSCDQN TDVQYNRCDI PEETWTGLE3. H3 trimethyl K4 peptide
ARTXQTARKS TGGKAPRKQLSolvent system 90% H2O/10% D2O, Pressure 1 (±0.001) atm, Temperature 298 (±0.1) K, pH 7.0 (±0.05), Details 20mM d-Tris-HCl(pH {7.0);} 300uM {H3 peptide;} 100mM {NaCl;} 1mM {d-DTT;} 0.02% {NaN3;} 50uM ZnCl2+1mM {IDA;} 10% D2O, 90% H2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | zf-CW domain | protein | 0.0 ~ 0.0 mM | |
2 | H3 trimethyl K4 peptide | protein | 0.0 ~ 0.0 mM | |
3 | d-Tris-HCl | natural abundance | buffer | 20 mM |
4 | NaCl | natural abundance | salt | 100 mM |
5 | d-DTT | natural abundance | salt | 1 mM |
6 | NaN3 | natural abundance | salt | 0.02 % |
7 | ZnCl2 | natural abundance | salt | 50 uM |
8 | IDA | natural abundance | salt | 1 mM |
9 | H2O | solvent | 90 % | |
10 | D2O | solvent | 10 % |