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Found 1 Document (1.055 seconds)

BMRB: 11423

2RRL

Solution structure of the C-terminal domain of the FliK

Authors

Mizuno, S., Tate, S., Kobayashi, N., Amida, H.

Assembly

Flagellar hook-length control protein

Entity

1. Flagellar hook-length control protein (polymer, Thiol state: not present), 169 monomers, 17758.64 Da Detail

HMASDDRATG PALTPLVVAA AATSAKVEVD SPPAPVTHGA AMPTLSSATA QPLPVASAPV LSAPLGSHEW QQTFSQQVML FTRQGQQSAQ LRLHPEELGQ VHISLKLDDN QAQLQMVSPH SHVRAALEAA LPMLRTQLAE SGIQLGQSSI SSESFAGQQQ SSSQQQSSR

Formula weight

17758.64 Da

Source organism

Salmonella enterica subsp. enterica serovar Typhimurium

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.8 %, Completeness: 94.2 %, Completeness (bb): 96.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	94.2 % (1737 of 1843)	93.8 % (894 of 953)	95.6 % (679 of 710)	91.1 % (164 of 180)
Backbone	96.9 % (957 of 988)	96.7 % (322 of 333)	97.6 % (487 of 499)	94.9 % (148 of 156)
Sidechain	92.4 % (939 of 1016)	92.3 % (572 of 620)	94.4 % (351 of 372)	66.7 % (16 of 24)
Aromatic	74.3 % (52 of 70)	80.0 % (28 of 35)	67.6 % (23 of 34)	100.0 % (1 of 1)
Methyl	99.5 % (193 of 194)	100.0 % (97 of 97)	99.0 % (96 of 97)

1. UNP residues 204-370

HMASDDRATG PALTPLVVAA AATSAKVEVD SPPAPVTHGA AMPTLSSATA QPLPVASAPV LSAPLGSHEW QQTFSQQVML FTRQGQQSAQ LRLHPEELGQ VHISLKLDDN QAQLQMVSPH SHVRAALEAA LPMLRTQLAE SGIQLGQSSI SSESFAGQQQ SSSQQQSSR

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
1	UNP residues 204-370	[U-13C; U-15N]	protein	1 mM
2	sodium phosphate	natural abundance	buffer	50 mM
3	EDTA	natural abundance	buffer	1 mM
4	H2O		solvent	90 %
5	D2O		solvent	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
6	UNP residues 204-370	[U-13C; U-15N]	protein	1 mM
7	sodium phosphate	natural abundance	buffer	50 mM
8	EDTA	natural abundance	buffer	1 mM
9	D2O		solvent	100 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
10	UNP residues 204-370	[U-15N]	protein	1 mM
11	sodium phosphate	natural abundance	buffer	50 mM
12	EDTA	natural abundance	buffer	1 mM
13	H2O		solvent	90 %
14	D2O		solvent	10 %

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
15	UNP residues 204-370	[U-15N]	protein	0.5 mM
16	sodium phosphate	natural abundance	buffer	50 mM
17	EDTA	natural abundance	buffer	1 mM
18	D2O		solvent	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.21 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 15 models in PDB: 2RRL, Strand ID: A Detail

Release date

2011-06-21

Citation

The NMR structure of FliK, the trigger for the switch of substrate specificity in the flagellar type III secretion apparatus
Mizuno, S., Amida, H., Kobayashi, N., Aizawa, S., Tate, S.
J. Mol. Biol. (2011), 409, 558-573, PubMed 21510958 , DOI 10.1016/j.jmb.2011.04.008 , Abstract

Related entities 1. Flagellar hook-length control protein, : 1 : 1 : 10 entities Detail

Interaction partners 1. Flagellar hook-length control protein, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 27 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
1	UNP residues 204-370	[U-13C; U-15N]	protein	1 mM
2	sodium phosphate	natural abundance	buffer	50 mM
3	EDTA	natural abundance	buffer	1 mM
4	H2O		solvent	90 %
5	D2O		solvent	10 %

2 2D 1H-13C HSQC aliphatic

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
1	UNP residues 204-370	[U-13C; U-15N]	protein	1 mM
2	sodium phosphate	natural abundance	buffer	50 mM
3	EDTA	natural abundance	buffer	1 mM
4	H2O		solvent	90 %
5	D2O		solvent	10 %

3 2D 1H-13C HSQC aromatic

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
1	UNP residues 204-370	[U-13C; U-15N]	protein	1 mM
2	sodium phosphate	natural abundance	buffer	50 mM
3	EDTA	natural abundance	buffer	1 mM
4	H2O		solvent	90 %
5	D2O		solvent	10 %

4 3D HNCO

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
1	UNP residues 204-370	[U-13C; U-15N]	protein	1 mM
2	sodium phosphate	natural abundance	buffer	50 mM
3	EDTA	natural abundance	buffer	1 mM
4	H2O		solvent	90 %
5	D2O		solvent	10 %

5 3D HNCA

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
1	UNP residues 204-370	[U-13C; U-15N]	protein	1 mM
2	sodium phosphate	natural abundance	buffer	50 mM
3	EDTA	natural abundance	buffer	1 mM
4	H2O		solvent	90 %
5	D2O		solvent	10 %

6 3D HN(CO)CA

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
1	UNP residues 204-370	[U-13C; U-15N]	protein	1 mM
2	sodium phosphate	natural abundance	buffer	50 mM
3	EDTA	natural abundance	buffer	1 mM
4	H2O		solvent	90 %
5	D2O		solvent	10 %

7 3D HCACO

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
1	UNP residues 204-370	[U-13C; U-15N]	protein	1 mM
2	sodium phosphate	natural abundance	buffer	50 mM
3	EDTA	natural abundance	buffer	1 mM
4	H2O		solvent	90 %
5	D2O		solvent	10 %

8 3D HNCACB

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
1	UNP residues 204-370	[U-13C; U-15N]	protein	1 mM
2	sodium phosphate	natural abundance	buffer	50 mM
3	EDTA	natural abundance	buffer	1 mM
4	H2O		solvent	90 %
5	D2O		solvent	10 %

9 3D CBCA(CO)NH

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
1	UNP residues 204-370	[U-13C; U-15N]	protein	1 mM
2	sodium phosphate	natural abundance	buffer	50 mM
3	EDTA	natural abundance	buffer	1 mM
4	H2O		solvent	90 %
5	D2O		solvent	10 %

10 3D C(CO)NH

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
1	UNP residues 204-370	[U-13C; U-15N]	protein	1 mM
2	sodium phosphate	natural abundance	buffer	50 mM
3	EDTA	natural abundance	buffer	1 mM
4	H2O		solvent	90 %
5	D2O		solvent	10 %

11 3D HNCANNH

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
1	UNP residues 204-370	[U-13C; U-15N]	protein	1 mM
2	sodium phosphate	natural abundance	buffer	50 mM
3	EDTA	natural abundance	buffer	1 mM
4	H2O		solvent	90 %
5	D2O		solvent	10 %

12 3D HBHA(CO)NH

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
1	UNP residues 204-370	[U-13C; U-15N]	protein	1 mM
2	sodium phosphate	natural abundance	buffer	50 mM
3	EDTA	natural abundance	buffer	1 mM
4	H2O		solvent	90 %
5	D2O		solvent	10 %

13 3D HCCH-TOCSY

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
1	UNP residues 204-370	[U-13C; U-15N]	protein	1 mM
2	sodium phosphate	natural abundance	buffer	50 mM
3	EDTA	natural abundance	buffer	1 mM
4	H2O		solvent	90 %
5	D2O		solvent	10 %

14 3D HCCH-COSY

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
1	UNP residues 204-370	[U-13C; U-15N]	protein	1 mM
2	sodium phosphate	natural abundance	buffer	50 mM
3	EDTA	natural abundance	buffer	1 mM
4	H2O		solvent	90 %
5	D2O		solvent	10 %

15 3D 1H-13C dec NOESY

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
1	UNP residues 204-370	[U-13C; U-15N]	protein	1 mM
2	sodium phosphate	natural abundance	buffer	50 mM
3	EDTA	natural abundance	buffer	1 mM
4	H2O		solvent	90 %
5	D2O		solvent	10 %

16 3D 1H-13C NOESY aliphatic

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
1	UNP residues 204-370	[U-13C; U-15N]	protein	1 mM
2	sodium phosphate	natural abundance	buffer	50 mM
3	EDTA	natural abundance	buffer	1 mM
4	H2O		solvent	90 %
5	D2O		solvent	10 %

17 3D 1H-13C NOESY aromatic

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
1	UNP residues 204-370	[U-13C; U-15N]	protein	1 mM
2	sodium phosphate	natural abundance	buffer	50 mM
3	EDTA	natural abundance	buffer	1 mM
4	H2O		solvent	90 %
5	D2O		solvent	10 %

18 2D 1H-13C HSQC aliphatic

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
6	UNP residues 204-370	[U-13C; U-15N]	protein	1 mM
7	sodium phosphate	natural abundance	buffer	50 mM
8	EDTA	natural abundance	buffer	1 mM
9	D2O		solvent	100 %

19 3D HCCH-TOCSY

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
6	UNP residues 204-370	[U-13C; U-15N]	protein	1 mM
7	sodium phosphate	natural abundance	buffer	50 mM
8	EDTA	natural abundance	buffer	1 mM
9	D2O		solvent	100 %

20 3D HCCH-COSY

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
6	UNP residues 204-370	[U-13C; U-15N]	protein	1 mM
7	sodium phosphate	natural abundance	buffer	50 mM
8	EDTA	natural abundance	buffer	1 mM
9	D2O		solvent	100 %

21 3D 1H-13C NOESY aliphatic

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
6	UNP residues 204-370	[U-13C; U-15N]	protein	1 mM
7	sodium phosphate	natural abundance	buffer	50 mM
8	EDTA	natural abundance	buffer	1 mM
9	D2O		solvent	100 %

22 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
10	UNP residues 204-370	[U-15N]	protein	1 mM
11	sodium phosphate	natural abundance	buffer	50 mM
12	EDTA	natural abundance	buffer	1 mM
13	H2O		solvent	90 %
14	D2O		solvent	10 %

23 3D 1H-15N NOESY

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
10	UNP residues 204-370	[U-15N]	protein	1 mM
11	sodium phosphate	natural abundance	buffer	50 mM
12	EDTA	natural abundance	buffer	1 mM
13	H2O		solvent	90 %
14	D2O		solvent	10 %

24 3D 1H-15N TOCSY

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
10	UNP residues 204-370	[U-15N]	protein	1 mM
11	sodium phosphate	natural abundance	buffer	50 mM
12	EDTA	natural abundance	buffer	1 mM
13	H2O		solvent	90 %
14	D2O		solvent	10 %

25 3D HNHA

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
10	UNP residues 204-370	[U-15N]	protein	1 mM
11	sodium phosphate	natural abundance	buffer	50 mM
12	EDTA	natural abundance	buffer	1 mM
13	H2O		solvent	90 %
14	D2O		solvent	10 %

26 2D 1H-1H NOESY

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
15	UNP residues 204-370	[U-15N]	protein	0.5 mM
16	sodium phosphate	natural abundance	buffer	50 mM
17	EDTA	natural abundance	buffer	1 mM
18	D2O		solvent	100 %

27 2D 1H-1H TOCSY

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
15	UNP residues 204-370	[U-15N]	protein	0.5 mM
16	sodium phosphate	natural abundance	buffer	50 mM
17	EDTA	natural abundance	buffer	1 mM
18	D2O		solvent	100 %

nullKeywords bacterial flagella motor, FlhB, FliK, hook-length control, NMR, protein transport

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Found 1 Document (1.055 seconds)

BMRB: 11423

Sample #1

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Sample #4

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Flagellar hook-length control protein, : 1 : 1 : 10 entities Detail

Interaction partners 1. Flagellar hook-length control protein, : 1 interactors Detail

Experiments performed 27 experiments Detail

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