Solution structure of Magnesium-bound form of calmodulin C-domain E104D/E140D mutant
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 94.1 % (756 of 803) | 94.0 % (391 of 416) | 96.1 % (298 of 310) | 87.0 % (67 of 77) |
Backbone | 94.7 % (409 of 432) | 96.0 % (143 of 149) | 94.8 % (200 of 211) | 91.7 % (66 of 72) |
Sidechain | 94.5 % (414 of 438) | 92.9 % (248 of 267) | 99.4 % (165 of 166) | 20.0 % (1 of 5) |
Aromatic | 100.0 % (50 of 50) | 100.0 % (25 of 25) | 100.0 % (25 of 25) | |
Methyl | 100.0 % (66 of 66) | 100.0 % (33 of 33) | 100.0 % (33 of 33) |
1. entity
MDTDSEEEIR EAFRVFDKDG NGYISAADLR HVMTNLGEKL TDEEVDEMIR EADIDGDGQV NYEDFVQMMT AKSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | MES | [U-2H] | 10 mM | |
2 | potassium chloride | natural abundance | 100 mM | |
3 | magnesium chloride | natural abundance | 100 mM | |
4 | H2O | natural abundance | 90 % | |
5 | D2O | natural abundance | 10 % |