NMR strucure of stereo-array isotope labelled (SAIL) peptidyl-prolyl cis-trans isomerase from E. coli (EPPIb)
MVTFHTNHGD IVIKTFDDKA PETVKNFLDY CREGFYNNTI FHRVINGFMI QGGGFEPGMK QKATKEPIKN EANNGLKNTR GTLAMARTQA PHSATAQFFI NVVDNDFLNF SGESLQGWGY CVFAEVVDGM DVVDKIKGVA TGRSGMHQDV PKEDVIIESV TVSE
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 76.6 % (1441 of 1882) | 76.4 % (742 of 971) | 71.6 % (525 of 733) | 97.8 % (174 of 178) |
Backbone | 83.7 % (815 of 974) | 95.6 % (324 of 339) | 69.7 % (332 of 476) | 100.0 % (159 of 159) |
Sidechain | 73.1 % (772 of 1056) | 66.1 % (418 of 632) | 83.7 % (339 of 405) | 78.9 % (15 of 19) |
Aromatic | 74.4 % (131 of 176) | 86.4 % (76 of 88) | 62.1 % (54 of 87) | 100.0 % (1 of 1) |
Methyl | 78.0 % (131 of 168) | 79.8 % (67 of 84) | 76.2 % (64 of 84) |
1. entity
MVTFHTNHGD IVIKTFDDKA PETVKNFLDY CREGFYNNTI FHRVINGFMI QGGGFEPGMK QKATKEPIKN EANNGLKNTR GTLAMARTQA PHSATAQFFI NVVDNDFLNF SGESLQGWGY CVFAEVVDGM DVVDKIKGVA TGRSGMHQDV PKEDVIIESV TVSESolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | EPPIb | SAIL [e-SAIL Phe, e-SAIL Tyr] | 0.3 mM | |
2 | sodium phosphate | natural abundance | 50 mM | |
3 | DTT | natural abundance | 1 mM | |
4 | sodium chloride | natural abundance | 100 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | EPPIb | SAIL [z-SAIL Phe, e-SAIL Tyr] | 0.3 mM | |
6 | sodium phosphate | natural abundance | 50 mM | |
7 | DTT | natural abundance | 1 mM | |
8 | sodium chloride | natural abundance | 100 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
9 | EPPIb | SAIL [d-SAIL Phe, d-SAIL Tyr] | 0.3 mM | |
10 | sodium phosphate | natural abundance | 50 mM | |
11 | DTT | natural abundance | 1 mM | |
12 | sodium chloride | natural abundance | 100 mM |