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BMRB: 11452

2LIY

NMR chemical shift assignments for stomagen from Arabidopsis thaliana

Authors

Ohki, S., Takeuchi, M., Mori, M.

Assembly

stomagen

Entity

1. stomagen (polymer, Thiol state: all disulfide bound), 45 monomers, 5122.717 Da Detail

IGSTAPTCTY NECRGCRYKC RAEQVPVEGN DPINSAYHYR CVCHR

Formula weight

5122.717 Da

Entity Connection

disulfide 3 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS8:SG	1:CYS41:SG
2	disulfide	sing	1:CYS13:SG	1:CYS20:SG
3	disulfide	sing	1:CYS16:SG	1:CYS43:SG

Source organism

Arabidopsis thaliana

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 85.3 %, Completeness (bb): 94.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	85.3 % (422 of 495)	86.5 % (224 of 259)	80.5 % (153 of 190)	97.8 % (45 of 46)
Backbone	94.7 % (250 of 264)	93.3 % (84 of 90)	94.7 % (125 of 132)	97.6 % (41 of 42)
Sidechain	77.7 % (212 of 273)	82.8 % (140 of 169)	68.0 % (68 of 100)	100.0 % (4 of 4)
Aromatic	25.0 % (10 of 40)	50.0 % (10 of 20)	0.0 % (0 of 20)
Methyl	84.4 % (27 of 32)	93.8 % (15 of 16)	75.0 % (12 of 16)

1. stomagen peptide

IGSTAPTCTY NECRGCRYKC RAEQVPVEGN DPINSAYHYR CVCHR

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.8

#	Name	Isotope labeling	Type	Concentration
1	Stomagen	natural abundance	protein	0.5 ~ 0.6 mM
2	sodium phosphate	natural abundance		10 mM
3	potassium chloride	natural abundance		100 mM
4	sodium azide	natural abundance		0.02 %
5	H2O	natural abundance	solvent	90 %
6	D2O	natural abundance	solvent	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 5.8

#	Name	Isotope labeling	Type	Concentration
7	Stomagen	natural abundance	protein	0.5 ~ 0.6 mM
8	sodium phosphate	natural abundance		10 mM
9	potassium chloride	natural abundance		100 mM
10	sodium azide	natural abundance		0.02 %
11	D2O	natural abundance	solvent	100 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.8

#	Name	Isotope labeling	Type	Concentration
12	Stomagen	[U-15N]	protein	0.1 ~ 0.2 mM
13	sodium phosphate	natural abundance		10 mM
14	potassium chloride	natural abundance		100 mM
15	sodium azide	natural abundance		0.02 %
16	H2O	natural abundance	solvent	90 %
17	D2O	natural abundance	solvent	10 %

Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.8

#	Name	Isotope labeling	Type	Concentration
18	Stomagen	[U-13C; U-15N]	protein	0.3 ~ 0.4 mM
19	sodium phosphate	natural abundance		10 mM
20	potassium chloride	natural abundance		100 mM
21	sodium azide	natural abundance		0.02 %
22	H2O	natural abundance	solvent	90 %
23	D2O	natural abundance	solvent	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	0.0 ppm	na	indirect	0.2514495
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	water	protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	0.0 ppm	na	indirect	0.2514495
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	water	protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	0.0 ppm	na	indirect	0.2514495
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	water	protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	0.0 ppm	na	indirect	0.2514495
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	water	protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: 0.28 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LIY, Strand ID: A Detail

Release date

2012-08-06

Citation

The NMR structure of stomagen reveals the basis of stomatal density regulation by plant peptide hormones
Ohki, S., Takeuchi, M., Mori, M.
Nat. Commun. (2011), 2, 512-512, PubMed 22027592 , DOI 10.1038/ncomms1520 , Abstract