BMRB: 11477

Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT

Authors

Tochio, N., Umehara, T., Kigawa, T., Yokoyama, S.

Assembly

protein

Entity

1. protein (polymer, Thiol state: all other bound), 36 monomers, 4190.661 Da Detail

GSSGSSGLLY DCHICERKFK NELDRDRHML VHGDKW

2. ZINC ION (non-polymer), 65.409 Da

Total weight

4256.0703 Da

Max. entity weight

4190.661 Da

Entity Connection

na 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS12:SG	2:ZN1:ZN
2	na	sing	1:CYS15:SG	2:ZN1:ZN
3	na	sing	1:HIS28:NE2	2:ZN1:ZN
4	na	sing	1:HIS32:NE2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 83.3 %, Completeness: 87.8 %, Completeness (bb): 81.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	87.8 % (366 of 417)	87.7 % (192 of 219)	90.0 % (144 of 160)	78.9 % (30 of 38)
Backbone	81.0 % (175 of 216)	77.6 % (59 of 76)	84.6 % (88 of 104)	77.8 % (28 of 36)
Sidechain	94.0 % (219 of 233)	93.0 % (133 of 143)	95.5 % (84 of 88)	100.0 % (2 of 2)
Aromatic	100.0 % (42 of 42)	100.0 % (21 of 21)	100.0 % (20 of 20)	100.0 % (1 of 1)
Methyl	100.0 % (24 of 24)	100.0 % (12 of 12)	100.0 % (12 of 12)

1. protein

GSSGSSGLLY DCHICERKFK NELDRDRHML VHGDKW

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 296 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	protein	[U-13C; U-15N]	protein	1.09 mM
2	TRIS	[U-2H]		20 mM
3	sodium chloride	natural abundance		100 mM
4	DTT	[U-2H]		1 mM
5	sodium azide	natural abundance		0.02 %
6	zinc chloride	natural abundance		50 uM
7	H2O		solvent	90 %
8	D2O		solvent	10 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 2RUX, Strand ID: A Detail

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Release date

2015-01-19

Citation

Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT
Tochio, N., Umehara, T., Kigawa, T., Yokoyama, S.
J. Struct. Funct. Genomics (2015), 16, 55-65, PubMed 25801860 , DOI 10.1007/s10969-015-9196-3 , Abstract

Related entities 1. protein, : 1 : 1 : 5 : 116 entities Detail

Interaction partners 1. protein, : 2 interactors Detail

Experiments performed 2 experiments Detail