Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | na | sing | 1:CYS12:SG | 2:ZN1:ZN |
2 | na | sing | 1:CYS15:SG | 2:ZN1:ZN |
3 | na | sing | 1:HIS28:NE2 | 2:ZN1:ZN |
4 | na | sing | 1:HIS33:NE2 | 2:ZN1:ZN |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 84.9 % (361 of 425) | 82.9 % (184 of 222) | 89.8 % (149 of 166) | 75.7 % (28 of 37) |
Backbone | 82.9 % (184 of 222) | 79.5 % (62 of 78) | 87.9 % (94 of 107) | 75.7 % (28 of 37) |
Sidechain | 87.7 % (207 of 236) | 84.7 % (122 of 144) | 92.4 % (85 of 92) | |
Aromatic | 95.0 % (38 of 40) | 95.0 % (19 of 20) | 95.0 % (19 of 20) | |
Methyl | 100.0 % (28 of 28) | 100.0 % (14 of 14) | 100.0 % (14 of 14) |
1. entity 1
GSSGSSGEKF ACDYCSFTCL SKGHLKVHIE RVHKKIKSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 296 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | protein | [U-13C; U-15N] | protein | 1.09 mM |
2 | TRIS | [U-2H] | 20 mM | |
3 | sodium chloride | natural abundance | 100 mM | |
4 | DTT | [U-2H] | 1 mM | |
5 | sodium azide | natural abundance | 0.02 % | |
6 | zinc chloride | natural abundance | 50 uM | |
7 | H2O | solvent | 90 % | |
8 | D2O | solvent | 10 % |