RNA aptamer against prion protein in complex with the partial binding peptide
Polymer type: polyribonucleotide polypeptide(L)
Total | 1H | |
---|---|---|
All | 85.6 % (155 of 181) | 85.6 % (155 of 181) |
Backbone | 88.4 % (84 of 95) | 88.4 % (84 of 95) |
Sidechain | 82.6 % (71 of 86) | 82.6 % (71 of 86) |
Aromatic | 70.0 % (21 of 30) | 70.0 % (21 of 30) |
Methyl | 100.0 % (1 of 1) | 100.0 % (1 of 1) |
1. RNA (5'-R(*GP*GP*AP*GP*GP*AP*GP*GP*AP*GP*GP*A)-3')
GGAGGAGGAG GA2. P16
GQWNKPSKPK TNSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303 K, pH 6.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | RNA (5'-R(*GP*GP*AP*GP*GP*AP*GP*GP*AP*GP*GP*A)-3') | natural abundance | RNA | 1 mM |
2 | P16 | natural abundance | protein | 1 mM |
3 | potassium phosphate | natural abundance | 10 mM | |
4 | potassium chloride | natural abundance | 10 mM | |
5 | DSS | natural abundance | 0.01 mM | |
6 | sodium azide | natural abundance | 3 mM | |
7 | H2O | solvent | 95 % | |
8 | D2O | solvent | 5 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
9 | RNA (5'-R(*GP*GP*AP*GP*GP*AP*GP*GP*AP*GP*GP*A)-3') | natural abundance | RNA | 1 mM |
10 | P16 | natural abundance | protein | 1 mM |
11 | potassium phosphate | natural abundance | 10 mM | |
12 | potassium chloride | natural abundance | 10 mM | |
13 | DSS | natural abundance | 0.01 mM | |
14 | sodium azide | natural abundance | 3 mM | |
15 | D2O | solvent | 100 % |