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BMRB: 11490

6KJN

Backbone and side-chain 1H, 15N and 13C resonance assignments of the microtubule-binding domains of yeast cytoplasmic dynein in the high affinity state

Authors

Takarada, O.

Assembly

Microtubule-binding domain

Entity

1. Microtubule-binding domain (polymer, Thiol state: free and disulfide bound), 141 monomers, 16478.69 Da Detail

GSHMKSIQDC EPTILEAQRG VKNIKKQQLT EIRSMVNPPS GVKIVMEAVC AILGYQFSNW RDIQQFIRKD DFIHNIVHYD TTLHMKPQIR KYMEEEFLSD PNFTYETINR ASKACGPLYQ WVNAQINFSK CLENVDPLRQ E

Formula weight

16478.69 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS10:SG	1:CYS131:SG

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.3 %, Completeness: 75.6 %, Completeness (bb): 78.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	75.6 % (1303 of 1723)	79.1 % (717 of 907)	69.8 % (461 of 660)	80.1 % (125 of 156)
Backbone	78.6 % (654 of 832)	92.1 % (258 of 280)	65.6 % (274 of 418)	91.0 % (122 of 134)
Sidechain	75.6 % (776 of 1027)	73.2 % (459 of 627)	83.1 % (314 of 378)	13.6 % (3 of 22)
Aromatic	47.1 % (66 of 140)	50.0 % (35 of 70)	42.6 % (29 of 68)	100.0 % (2 of 2)
Methyl	97.9 % (137 of 140)	97.1 % (68 of 70)	98.6 % (69 of 70)

1. dynein microtubule-binding domain

GSHMKSIQDC EPTILEAQRG VKNIKKQQLT EIRSMVNPPS GVKIVMEAVC AILGYQFSNW RDIQQFIRKD DFIHNIVHYD TTLHMKPQIR KYMEEEFLSD PNFTYETINR ASKACGPLYQ WVNAQINFSK CLENVDPLRQ E

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	dynein microtubule-binding domain	[U-100% 13C; U-100% 15N]	protein	0.5 mM
2	potassium phosphate	natural abundance		10 mM
3	sodium chloride	natural abundance		200 mM
4	H2O		solvent	90 %
5	D2O		solvent	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
6	dynein microtubule-binding domain	[U-100% 13C; U-100% 15N]	protein	0.5 mM
7	potassium phosphate	natural abundance		10 mM
8	sodium chloride	natural abundance		200 mM
9	D2O		solvent	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 2.61 ppm, Outliers: 4 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 2.61 ppm, Outliers: 4 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 1 Detail

Release date

2014-01-13

Citation

Backbone and side-chain ¹H, ¹⁵N, and ¹³C resonance assignments of the microtubule-binding domain of yeast cytoplasmic dynein in the high and low-affinity states
Takarada, O., Nishida, N., Kikkawa, M., Shimada, I.
Biomol. NMR Assign. (2014), 8, 379-382, PubMed 23975349 , DOI 10.1007/s12104-013-9522-2 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 11495 released on 2014-01-13
Title Backbone and side-chain 1H, 15N and 13C resonance assignments of the microtubule-binding domains of yeast cytoplasmic dynein in the low affinity state

Related entities 1. Microtubule-binding domain, : 1 : 4 : 90 entities Detail

Interaction partners 1. Microtubule-binding domain, : 7 interactors Detail

More details for 7 interactors identified by 5 citations

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 500 MHz The cryo probe is equipped with to this spectrometer.

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	dynein microtubule-binding domain	[U-100% 13C; U-100% 15N]	protein	0.5 mM
2	potassium phosphate	natural abundance		10 mM
3	sodium chloride	natural abundance		200 mM
4	H2O		solvent	90 %
5	D2O		solvent	10 %

2 2D 1H-13C HSQC aliphatic