Backbone 1H, 13C, and 15N Chemical Shift Assignments for the peptidyl prolyl cis-trans isomerase domain of C113D mutant human Pin1 without sulfate ion
GSHMEPARVR CSHLLVKHSQ SRRPSSWRQE KITRTKEEAL ELINGYIQKI KSGEEDFESL ASQFSDDSSA KARGDLGAFS RGQMQKPFED ASFALRTGEM SGPVFTDSGI HIILRTE
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 44.1 % (594 of 1346) | 20.9 % (148 of 708) | 65.6 % (339 of 517) | 88.4 % (107 of 121) |
Backbone | 78.2 % (543 of 694) | 47.7 % (114 of 239) | 94.2 % (322 of 342) | 94.7 % (107 of 113) |
Sidechain | 19.6 % (149 of 760) | 7.0 % (33 of 469) | 41.0 % (116 of 283) | 0.0 % (0 of 8) |
Aromatic | 0.0 % (0 of 96) | 0.0 % (0 of 48) | 0.0 % (0 of 47) | 0.0 % (0 of 1) |
Methyl | 20.4 % (20 of 98) | 12.2 % (6 of 49) | 28.6 % (14 of 49) |
1. C113D mutant hPin1 PPIase domain
GSHMEPARVR CSHLLVKHSQ SRRPSSWRQE KITRTKEEAL ELINGYIQKI KSGEEDFESL ASQFSDDSSA KARGDLGAFS RGQMQKPFED ASFALRTGEM SGPVFTDSGI HIILRTESolvent system 94.12% H2O/5.88% D2O, Pressure 1 atm, Temperature 299 K, pH 6.8
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | C113D mutant hPin1 PPIase domain | [U-13C; U-15N] | protein | 0.2 mM |
2 | TRIS | natural abundance | buffer | 50 mM |
3 | DTT | natural abundance | 1 mM | |
4 | sodium azide | natural abundance | 0.03 % | |
5 | H2O | natural abundance | solvent | 94.12 % |
6 | D2O | natural abundance | solvent | 5.88 % |