BMRB: 11580

NMR Structures and Localization of Potential Fusion Peptides and Pre-transmembrane Region of SARS-CoV: Implications in Membrane Fusion

Authors

Mahajan, M., Bhattacharjya, S.

Assembly

Fusion peptide in DPC micelles

Entity

1. Fusion peptide in DPC micelles (polymer, Thiol state: not present), 19 monomers, 2278.667 Da Detail

MWKTPTLKYF GGFNFSQIL

Formula weight

2278.667 Da

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.7 %, Completeness: 49.8 %, Completeness (bb): 53.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C
All	49.8 % (116 of 233)	75.8 % (100 of 132)	15.8 % (16 of 101)
Backbone	53.2 % (50 of 94)	87.2 % (34 of 39)	29.1 % (16 of 55)
Sidechain	42.3 % (66 of 156)	71.0 % (66 of 93)	0.0 % (0 of 63)
Aromatic	38.8 % (19 of 49)	76.0 % (19 of 25)	0.0 % (0 of 24)
Methyl	50.0 % (8 of 16)	100.0 % (8 of 8)	0.0 % (0 of 8)

1. Fusion Peptide

MWKTPTLKYF GGFNFSQIL

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Temperature 315 K, pH 4.7, Details Fusion peptide in DPC micelles

#	Name	Isotope labeling	Type	Concentration
1	DPC	[U-99% 2H]		125 mM
2	Fusion Peptide	natural abundance	peptide	0.5 mM
3	H2O	natural abundance	solvent	90 %
4	D2O	[U-100% 2H]	solvent	10 %

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Calculated from 20 models in PDB: 2RUM, Strand ID: A Detail

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Release date

2016-07-12

Citation

NMR structures and localization of the potential fusion peptides and the pre-transmembrane region of SARS-CoV: Implications in membrane fusion
Mahajan, M., Bhattacharjya, S.
Biochim. Biophys. Acta (2015), 1848, 721-730, PubMed 25475644 , DOI 10.1016/j.bbamem.2014.11.025 , Abstract

Entries sharing articles BMRB: 2 entries Detail

Related entities 1. Fusion peptide in DPC micelles, : 1 : 13 : 6 entities Detail

Experiments performed 3 experiments Detail