Solution structure of Human Pin1 PPIase C113S mutant
GSHMEPARVR CSHLLVKHSQ SRRPSSWRQE KITRTKEEAL ELINGYIQKI KSGEEDFESL ASQFSDSSSA KARGDLGAFS RGQMQKPFED ASFALRTGEM SGPVFTDSGI HIILRTE
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 97.8 % (1316 of 1346) | 97.6 % (691 of 708) | 98.5 % (509 of 517) | 95.9 % (116 of 121) |
Backbone | 96.8 % (672 of 694) | 96.2 % (230 of 239) | 97.7 % (334 of 342) | 95.6 % (108 of 113) |
Sidechain | 98.7 % (750 of 760) | 98.3 % (461 of 469) | 99.3 % (281 of 283) | 100.0 % (8 of 8) |
Aromatic | 100.0 % (96 of 96) | 100.0 % (48 of 48) | 100.0 % (47 of 47) | 100.0 % (1 of 1) |
Methyl | 100.0 % (98 of 98) | 100.0 % (49 of 49) | 100.0 % (49 of 49) |
1. entity
GSHMEPARVR CSHLLVKHSQ SRRPSSWRQE KITRTKEEAL ELINGYIQKI KSGEEDFESL ASQFSDSSSA KARGDLGAFS RGQMQKPFED ASFALRTGEM SGPVFTDSGI HIILRTESolvent system 94% H2O/6% D2O, Pressure 1 atm, Temperature 299 K, pH 6.6
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | sodium sulfate | natural abundance | salt | 100 mM |
2 | sodium phosphate | natural abundance | buffer | 50 mM |
3 | D2O | [U-2H] | solvent | 6 % |
4 | H2O | natural abundance | solvent | 94 % |
5 | EDTA | natural abundance | 5 mM | |
6 | DTT | natural abundance | 1 mM | |
7 | NaN3 | natural abundance | 0.03 % |