Solution structure of the complex between XPC acidic domain and TFIIH p62 PH domain
GSMATSSEEV LLIVKKVRQK KQDGALYLMA ERIAWAPEGK DRFTISHMYA DIKCQKISPE GKAKIQLQLV LHAGDTTNFH FSNESTAVKE RDAVKDLLQQ LLPKFKRKAN
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 31.8 % (600 of 1886) | 29.1 % (288 of 989) | 33.1 % (240 of 724) | 41.6 % (72 of 173) |
Backbone | 37.8 % (364 of 964) | 36.9 % (121 of 328) | 36.6 % (175 of 478) | 43.0 % (68 of 158) |
Sidechain | 27.2 % (293 of 1076) | 25.4 % (168 of 661) | 30.3 % (121 of 400) | 26.7 % (4 of 15) |
Aromatic | 28.1 % (32 of 114) | 28.1 % (16 of 57) | 27.3 % (15 of 55) | 50.0 % (1 of 2) |
Methyl | 25.3 % (40 of 158) | 24.1 % (19 of 79) | 26.6 % (21 of 79) |
1. XPC acidic domain
GSHMAHHLKR GATMNEDSNE EEEESENDWE EVEELSEPVL GDVRESTAFS RS2. TFIIH p62 PH domain
GSMATSSEEV LLIVKKVRQK KQDGALYLMA ERIAWAPEGK DRFTISHMYA DIKCQKISPE GKAKIQLQLV LHAGDTTNFH FSNESTAVKE RDAVKDLLQQ LLPKFKRKANSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 6.8
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | XPC acidic domain | [U-99% 13C; U-99% 15N] | protein | 0.4 mM |
2 | TFIIH p62 PH domain | natural abundance | protein | 0.48 mM |
3 | potassium phosphate | natural abundance | buffer | 20 mM |
4 | DTT | [U-2H] | 5 mM | |
5 | H2O | natural abundance | solvent | 90 % |
6 | D2O | [U-2H] | solvent | 10 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 305 K, pH 6.8
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | XPC acidic domain | [U-99% 13C; U-99% 15N] | protein | 0.4 mM |
8 | TFIIH p62 PH domain | natural abundance | protein | 0.48 mM |
9 | potassium phosphate | natural abundance | buffer | 20 mM |
10 | DTT | [U-2H] | 5 mM | |
11 | D2O | [U-2H] | solvent | 10 % |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 51.2 % (1932 of 3772) | 49.0 % (969 of 1978) | 53.0 % (768 of 1448) | 56.4 % (195 of 346) |
Backbone | 54.0 % (1042 of 1928) | 52.1 % (342 of 656) | 54.4 % (520 of 956) | 57.0 % (180 of 316) |
Sidechain | 49.3 % (1060 of 2152) | 47.6 % (629 of 1322) | 52.0 % (416 of 800) | 50.0 % (15 of 30) |
Aromatic | 40.4 % (92 of 228) | 41.2 % (47 of 114) | 39.1 % (43 of 110) | 50.0 % (2 of 4) |
Methyl | 52.5 % (166 of 316) | 51.9 % (82 of 158) | 53.2 % (84 of 158) |
1. XPC acidic domain
GSHMAHHLKR GATMNEDSNE EEEESENDWE EVEELSEPVL GDVRESTAFS RS2. TFIIH p62 PH domain
GSMATSSEEV LLIVKKVRQK KQDGALYLMA ERIAWAPEGK DRFTISHMYA DIKCQKISPE GKAKIQLQLV LHAGDTTNFH FSNESTAVKE RDAVKDLLQQ LLPKFKRKANSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 305 K, pH 6.8
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | XPC acidic domain | [U-99% 13C; U-99% 15N] | protein | 0.4 mM |
2 | TFIIH p62 PH domain | natural abundance | protein | 0.48 mM |
3 | potassium phosphate | natural abundance | buffer | 20 mM |
4 | DTT | [U-2H] | 5 mM | |
5 | H2O | natural abundance | solvent | 90 % |
6 | D2O | [U-2H] | solvent | 10 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 305 K, pH 6.8
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | XPC acidic domain | [U-99% 13C; U-99% 15N] | protein | 0.4 mM |
8 | TFIIH p62 PH domain | natural abundance | protein | 0.48 mM |
9 | potassium phosphate | natural abundance | buffer | 20 mM |
10 | DTT | [U-2H] | 5 mM | |
11 | D2O | [U-2H] | solvent | 10 % |