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BMRB: 15034

8GPX

1H,13C and 15N NMR Assignments of the Yellow Fever Envelope Protein Domain III

Authors

Volk, D.E., Gandham, S.HARI., May, F.J., Barrett, A.D.T., Gorenstein, D.G.

Assembly

YFED3 monomer

Entity

1. YFED3 monomer (polymer, Thiol state: all disulfide bound), 112 monomers, 11938.64 Da Detail

MSALTLKGTS YKMCTDKMSF VKNPTDTGHG TVVMQVKVPK GAPCKIPVIV ADDLTAAINK GILVTVNPIA STNDDEVLIE VNPPFGDSYI IVGTGDSRLT YQWHKEGSSI GK

Formula weight

11938.64 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS14:SG	1:CYS44:SG

Source organism

Yellow fever virus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.1 %, Completeness: 94.5 %, Completeness (bb): 95.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	94.5 % (1183 of 1252)	95.3 % (613 of 643)	92.7 % (460 of 496)	97.3 % (110 of 113)
Backbone	95.3 % (627 of 658)	96.5 % (219 of 227)	93.9 % (306 of 326)	97.1 % (102 of 105)
Sidechain	92.0 % (640 of 696)	93.3 % (388 of 416)	90.1 % (245 of 272)	87.5 % (7 of 8)
Aromatic	68.8 % (44 of 64)	75.0 % (24 of 32)	64.5 % (20 of 31)	0.0 % (0 of 1)
Methyl	95.8 % (136 of 142)	97.2 % (69 of 71)	94.4 % (67 of 71)

1. YFED3

MSALTLKGTS YKMCTDKMSF VKNPTDTGHG TVVMQVKVPK GAPCKIPVIV ADDLTAAINK GILVTVNPIA STNDDEVLIE VNPPFGDSYI IVGTGDSRLT YQWHKEGSSI GK

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5.8

#	Name	Isotope labeling	Type	Concentration
1	YFED3	[U-98% 13C; U-98% 15N]		0.1 ~ 0.3 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.34 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.34 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.53 ppm, Outliers: 6 Detail

Release date

2007-06-26

Citation

NMR assignments of the yellow fever virus envelope protein domain III
Volk, D.E., Gandham, S.H.A., May, F.J., Anderson, A., Barrett, A.D.T., Gorenstein, D.G.
Biomol. NMR Assign. (2007), 1, 49-50, PubMed 19636823 , DOI 10.1007/s12104-007-9000-9 , Abstract

Related entities 1. YFED3 monomer, : 1 : 2 : 9 : 166 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC