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BMRB: 15036

2IZ3

SOLUTION STRUCTURE OF HUMAN BETA-MICROSEMINOPROTEIN

Authors

Ghasriani, H., Teilum, K., Johnsson, Y., Fernlund, P., Drakenberg, T.

Assembly

beta-microseminoprotein

Entity

1. beta-microseminoprotein (polymer, Thiol state: all disulfide bound), 97 monomers, 10941.31 Da Detail

GGGSCYFIPN EGVPGDSTRK CMDLKGNKHP INSEWQTDNC ETCTCYETEI SCCTLVSTPV GYDKDNCQRI FKKEDCKYIV VEKKDPKKTC SVSEWII

Formula weight

10941.31 Da

Entity Connection

disulfide 5 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS5:SG	1:CYS53:SG
2	disulfide	sing	1:CYS21:SG	1:CYS45:SG
3	disulfide	sing	1:CYS40:SG	1:CYS76:SG
4	disulfide	sing	1:CYS43:SG	1:CYS52:SG
5	disulfide	sing	1:CYS67:SG	1:CYS90:SG

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.0 %, Completeness: 87.3 %, Completeness (bb): 97.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	87.3 % (970 of 1111)	88.5 % (515 of 582)	83.4 % (357 of 428)	97.0 % (98 of 101)
Backbone	97.4 % (557 of 572)	96.9 % (190 of 196)	97.5 % (277 of 284)	97.8 % (90 of 92)
Sidechain	80.0 % (503 of 629)	84.2 % (325 of 386)	72.6 % (170 of 234)	88.9 % (8 of 9)
Aromatic	36.3 % (29 of 80)	70.0 % (28 of 40)	0.0 % (0 of 38)	50.0 % (1 of 2)
Methyl	100.0 % (76 of 76)	100.0 % (38 of 38)	100.0 % (38 of 38)

1. hMSP

GGGSCYFIPN EGVPGDSTRK CMDLKGNKHP INSEWQTDNC ETCTCYETEI SCCTLVSTPV GYDKDNCQRI FKKEDCKYIV VEKKDPKKTC SVSEWII

Show sample condition

Sample #1

Pressure 1 atm, Temperature 310.0 K, pH 6.0, Details 90% WATER/10% D2O

#	Name	Isotope labeling	Concentration
1	entity	[U-95% 13C; U-95% 15N]	3.4 ~ 4.2 mM
2	H20		90 %
3	D20		10 %

Sample #2

Pressure 1 atm, Temperature 310.0 K, pH 6.0, Details 100% D2O

#	Name	Isotope labeling	Type	Concentration
4	entity	[U-95% 13C; U-95% 15N]		3.4 ~ 4.2 mM
5	D20			100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.18 ppm, Outliers: 5 Detail

Release date

2015-07-22

Citation

Solution structures of human and porcine beta-microseminoprotein
Ghasriani, H., Teilum, K., Johnsson, Y., Fernlund, P., Drakenberg, T.
J. Mol. Biol. (2006), 362, 502-515, PubMed 16930619 , DOI 10.1016/j.jmb.2006.07.029 , Abstract

Related entities 1. beta-microseminoprotein, : 1 : 3 : 21 entities Detail

Interaction partners 1. beta-microseminoprotein, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC