1H, 13C, and 15N resonance assignments of FK506-binding domain of Plasmodium falciparum FKBP35
MTTEQEFEKV ELTADGGVIK TILKKGDEGE ENIPKKGNEV TVHYVGKLES TGKVFDSSFD RNVPFKFHLE QGEVIKGWDI CVSSMRKNEK CLVRIESMYG YGDEGCGESI PGNSVLLFEI ELLSFRELEH HHHHH
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 89.1 % (1411 of 1583) | 92.2 % (761 of 825) | 84.0 % (519 of 618) | 93.6 % (131 of 140) |
Backbone | 94.2 % (757 of 804) | 94.3 % (265 of 281) | 94.1 % (368 of 391) | 93.9 % (124 of 132) |
Sidechain | 85.1 % (766 of 900) | 91.2 % (496 of 544) | 75.6 % (263 of 348) | 87.5 % (7 of 8) |
Aromatic | 37.7 % (52 of 138) | 75.4 % (52 of 69) | 0.0 % (0 of 68) | 0.0 % (0 of 1) |
Methyl | 96.9 % (126 of 130) | 96.9 % (63 of 65) | 96.9 % (63 of 65) |
1. pfFKBP35FKBD
MTTEQEFEKV ELTADGGVIK TILKKGDEGE ENIPKKGNEV TVHYVGKLES TGKVFDSSFD RNVPFKFHLE QGEVIKGWDI CVSSMRKNEK CLVRIESMYG YGDEGCGESI PGNSVLLFEI ELLSFRELEH HHHHHSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.8, Details 0.5 mM in a buffer containing 20 mM Na-PO4, 50 mM NaCl, 1 mM DTT, 0.1mM EDTA, 10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | pfFKBP35FKBD | [U-13C; U-15N] | 0.5 mM | |
2 | Na-PO4 | 20 mM | ||
3 | NaCl | 50 mM | ||
4 | DTT | 1 mM | ||
5 | EDTA | 0.1 mM | ||
6 | D2O | 10 % | ||
7 | H2O | 90 % |