BMRB: 15046

1H, 13C, and 15N resonance assignments of subunit F of the A1AO ATP synthase from Methanosarcina mazei Go1

Authors

Gayen, S., Vivekanandan, S., Biukovic, G., Gruber, G., Yoon, H.

Assembly

Subunit F of the A1AO ATP synthase from Methanosarcina mazei Go1

Entity

1. Subunit F of the A1AO ATP synthase from Methanosarcina mazei Go1 (polymer, Thiol state: not present), 110 monomers, 11944.51 Da Detail

MKHHHHHHPM ELAVIGKSEF VTGFRLAGIS KVYETPDIPA TESAVRSVLE DKSVGILVMH NDDIGNLPEV LRKNLNESVQ PTVVALGGSG SGSTSLREKI KQAVGVDLWK

Formula weight

11944.51 Da

Source organism

Methanosarcina mazei

Exptl. method

solution NMR

Refine. method

Distance constraints, Simulated annealing, Molecular dynamics, Torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.7 %, Completeness: 85.0 %, Completeness (bb): 90.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	85.0 % (1058 of 1245)	85.8 % (552 of 643)	82.7 % (405 of 490)	90.2 % (101 of 112)
Backbone	90.3 % (587 of 650)	90.2 % (203 of 225)	90.6 % (290 of 320)	89.5 % (94 of 105)
Sidechain	81.0 % (563 of 695)	83.5 % (349 of 418)	76.7 % (207 of 270)	100.0 % (7 of 7)
Aromatic	27.9 % (19 of 68)	52.9 % (18 of 34)	0.0 % (0 of 33)	100.0 % (1 of 1)
Methyl	94.9 % (131 of 138)	95.7 % (66 of 69)	94.2 % (65 of 69)

1. F subunit A type ATP synthase

MKHHHHHHPM ELAVIGKSEF VTGFRLAGIS KVYETPDIPA TESAVRSVLE DKSVGILVMH NDDIGNLPEV LRKNLNESVQ PTVVALGGSG SGSTSLREKI KQAVGVDLWK

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 288 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	F subunit A type ATP synthase	[U-13C; U-15N]		0.1 ~ 0.5 mM

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LACS Plot; CA

Referencing offset: 2.75 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 2.75 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.04 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 2.89 ppm, Outliers: 5 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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Protein Blocks Logo

Calculated from 10 models in PDB: 2OV6, Strand ID: A Detail

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Release date

2007-06-25

Citation

1H, 13C, and 15N resonance assignments of subunit F of the A(1)A (O) ATP synthase from Methanosarcina mazei Gö1
Gayen, S., Vivekanandan, S., Biukovic, G., Gruber, G., Yoon, H.
Biomol. NMR Assign. (2007), 1, 23-25, PubMed 19636817 , DOI 10.1007/s12104-007-9004-5 , Abstract

Related entities 1. Subunit F of the A1AO ATP synthase from Methanosarcina mazei Go1, : 1 : 1 : 34 entities Detail

Experiments performed 11 experiments Detail