BMRB: 15050

piscidin solution structure in presence of DPC micelles

Authors

Campagna, S., Saint, N., Molle, G., Aumelas, A.

Assembly

piscidin

Entity

1. piscidin (polymer, Thiol state: not present), 23 monomers, 2570.028 Da Detail

FFHHIFRGIV HVGKTIHRLV TGX

Formula weight

2570.028 Da

Source organism

Morone saxatilis

Exptl. method

solution NMR

Refine. method

distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.7 %, Completeness: 90.6 %, Completeness (bb): 93.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	90.6 % (126 of 139)	90.6 % (126 of 139)
Backbone	93.6 % (44 of 47)	93.6 % (44 of 47)
Sidechain	89.1 % (82 of 92)	89.1 % (82 of 92)
Aromatic	65.2 % (15 of 23)	65.2 % (15 of 23)
Methyl	100.0 % (16 of 16)	100.0 % (16 of 16)

1. piscidin-1

FFHHIFRGIV HVGKTIHRLV TGX

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.01) atm, Temperature 300 (±0.01) K, pH 5.0 (±0.01)

#	Name	Isotope labeling	Type	Concentration
1	piscidin-1	natural abundance		1 (±0.1) mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.01) atm, Temperature 300 (±0.01) K, pH 5.0 (±0.01), Details Piscidin is in presence of 39 mM of DPC

#	Name	Isotope labeling	Type	Concentration
2	piscidin-1	natural abundance		1 (±0.1) mM
3	DPC	natural abundance		39 mM

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Protein Blocks Logo

Calculated from 10 models in PDB: 2JOS, Strand ID: A Detail

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Release date

2015-11-15

Citation

Structure and mechanism of action of the antimicrobial peptide piscidin
Campagna, S., Saint, N., Molle, G., Aumelas, A.
Biochemistry (2007), 46, 1771-1778, PubMed 17253775 , DOI 10.1021/bi0620297 , Abstract

Related entities 1. piscidin, : 1 : 7 : 7 entities Detail

Experiments performed 6 experiments Detail

Chemical shift validation 3 contents Detail