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piscidin solution structure in presence of DPC micelles
Authors
Campagna, S., Saint, N., Molle, G., Aumelas, A.
Assembly
piscidin
Entity
1. piscidin (polymer, Thiol state: not present), 23 monomers, 2570.028 Da Detail

FFHHIFRGIV HVGKTIHRLV TGX


Formula weight
2570.028 Da
Source organism
Morone saxatilis
Exptl. method
solution NMR
Refine. method
distance geometry
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 95.7 %, Completeness: 90.6 %, Completeness (bb): 93.6 % Detail

Polymer type: polypeptide(L)

Total1H
All90.6 % (126 of 139)90.6 % (126 of 139)
Backbone93.6 % (44 of 47)93.6 % (44 of 47)
Sidechain89.1 % (82 of 92)89.1 % (82 of 92)
Aromatic65.2 % (15 of 23)65.2 % (15 of 23)
Methyl100.0 % (16 of 16)100.0 % (16 of 16)

1. piscidin-1

FFHHIFRGIV HVGKTIHRLV TGX

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.01) atm, Temperature 300 (±0.01) K, pH 5.0 (±0.01)


#NameIsotope labelingTypeConcentration
1piscidin-1natural abundance1 (±0.1) mM
Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.01) atm, Temperature 300 (±0.01) K, pH 5.0 (±0.01), Details Piscidin is in presence of 39 mM of DPC


#NameIsotope labelingTypeConcentration
2piscidin-1natural abundance1 (±0.1) mM
3DPCnatural abundance39 mM

Protein Blocks Logo
Calculated from 10 models in PDB: 2JOS, Strand ID: A Detail


Release date
2015-11-15
Citation
Structure and mechanism of action of the antimicrobial peptide piscidin
Campagna, S., Saint, N., Molle, G., Aumelas, A.
Biochemistry (2007), 46, 1771-1778, PubMed 17253775 , DOI 10.1021/bi0620297 ,
Entries sharing articles Swiss-Prot: 1 entries Detail
  Swiss-Prot: Q8UUG0 released on 2003-10-10
    Title MORO_MORSA Entity Moronecidin
Related entities 1. piscidin, : 1 : 7 : 7 entities Detail
Experiments performed 6 experiments Detail
Chemical shift validation 3 contents Detail
Keywords DPC micelles, NMR, alpha-helix, antimicrobial peptide