BMRBj - BMREntryMaster

Found 1 Document (0.638 seconds)

BMRB: 15097

Dynamics and Calcium-Dependent Actin Bundling by the two Carboxy Terminal Domains of Villin

Authors

Smirnov, S.L., Isern, N.G., Hoyt, D.W., Jiang, Z., McKnight, C.

Assembly

D6-HP

Entity

1. D6-HP (polymer, Thiol state: all free), 208 monomers, 23504.01 Da Detail

PRLFECSNKT GRFLATEIVD FTQDDLDEND VYLLDTWDQI FFWIGKGANE SEKEAAAETA QEYLRSHPGS RDLDTPIIVV KQGFEPPTFT GWFMAWDPLC WSDRKSYDEL KAELGDNASI GQLVSGLTSK NEVFTATTTL VPTKLETFPL DVLVNTAAED LPRGVDPSRK ENHLSDEDFK AVFGMTRSAF ANLPLWKQQN LKKEKGLF

Formula weight

23504.01 Da

Source organism

Gallus gallus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, heteronucl_NOEs, heteronucl_T1_relaxation, heteronucl_T2_relaxation, order_parameters

Chem. Shift Complete

Sequence coverage: 93.8 %, Completeness: 65.3 %, Completeness (bb): 80.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	65.3 % (1595 of 2443)	64.7 % (818 of 1265)	62.3 % (597 of 959)	82.2 % (180 of 219)
Backbone	80.0 % (981 of 1226)	89.4 % (373 of 417)	69.9 % (428 of 612)	91.4 % (180 of 197)
Sidechain	56.2 % (794 of 1413)	52.5 % (445 of 848)	64.3 % (349 of 543)	0.0 % (0 of 22)
Aromatic	9.8 % (24 of 244)	19.7 % (24 of 122)	0.0 % (0 of 116)	0.0 % (0 of 6)
Methyl	71.4 % (157 of 220)	70.9 % (78 of 110)	71.8 % (79 of 110)

1. D6-HP

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	D6-HP	[U-98% 13C; U-98% 15N]	1.1 (±0.1) mM
2	DTT	[U-2H]	10 mM
3	TSP	natural abundance	1 mM
4	PIPES	[U-2H]	20 mM
5	Ca2+	natural abundance	5 mM
6	NaN3	natural abundance	0.01 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	na	indirect	0.2514495
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	na	indirect	0.1013291

Referencing offset: -0.17 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	na	indirect	0.2514495
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	na	indirect	0.1013291

Referencing offset: -0.17 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	TSP	methyl protons	na	indirect	0.2514495
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	TSP	methyl protons	na	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 2 Detail

Heteronucl. T₁

144 T₁ values in 1 lists
Coherence Sz, Field strength (¹H) 750 MHz, Pressure 1 atm, Temperature 298 K, pH 7 Detail

Heteronucl. T₂

144 T₂ values in 1 lists
Coherence S(+,-), Field strength (¹H) 750 MHz, Pressure 1 atm, Temperature 298 K, pH 7 Detail

Heteronucl. NOE

144 NOE values in 1 lists
Value type peak height, Field strength (¹H) 750 MHz, Pressure 1 atm, Temperature 298 K, pH 7 Detail

Heteronucl. T₁/T₂

144 T₁/T₂ values in 1 lists
Field strength (¹H) 750 MHz, Pressure 1 atm, Temperature 298 K, pH 7 Detail

Order parameters

107 S² values in 1 lists
Pressure 1 atm, Temperature 298 K, pH 7 Detail

Release date

2007-08-20

Citation

The isolated sixth gelsolin repeat and headpiece domain of villin bundle F-actin in the presence of calcium and are linked by a 40-residue unstructured sequence
Smirnov, S.L., Isern, N.G., Jiang, Z.G., Hoyt, D.W., McKnight, C.
Biochemistry (2007), 46, 7488-7496, PubMed 17547371 , DOI 10.1021/bi700110v , Abstract

Related entities 1. D6-HP, : 1 : 39 entities Detail

Interaction partners 1. D6-HP, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 16 experiments Detail

1 2D 1H-15N HSQC