Solution Structure and Binding Property of the Domain-swapped Dimer of ZO2PDZ2
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 83.0 % (807 of 972) | 78.5 % (399 of 508) | 85.9 % (322 of 375) | 96.6 % (86 of 89) |
Backbone | 89.6 % (446 of 498) | 83.3 % (145 of 174) | 91.7 % (221 of 241) | 96.4 % (80 of 83) |
Sidechain | 78.5 % (431 of 549) | 76.0 % (254 of 334) | 81.8 % (171 of 209) | 100.0 % (6 of 6) |
Aromatic | 45.5 % (10 of 22) | 90.9 % (10 of 11) | 0.0 % (0 of 11) | |
Methyl | 89.5 % (102 of 114) | 86.0 % (49 of 57) | 93.0 % (53 of 57) |
1. ZO2PDZ2
MIGVLLMKSR ANEEYGLRLG SQIFVKEMTR TGLATKDGNL HEGDIILKIN GTVTENMSLT DARKLIEKSR GKLQLVVLRD SLESolvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5, Details 0.8mM ZO2PDZ2 domain, 20mM phosphate buffer (pH 6.5), 50mM NaCl, 1mM EDTA, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | ZO2PDZ2 | natural abundance | 0.8 (±0.1) mM | |
2 | phosphate buffer | natural abundance | 20 mM | |
3 | NaCl | natural abundance | 50 mM | |
4 | EDTA | natural abundance | 1 mM |
Solvent system 90% H2O, 10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5, Details 0.8mM 15N-labeled ZO2PDZ2 domain, 20mM phosphate buffer(pH 6.5), 50mM NaCl, 1mM EDTA, 90% H2O, 10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | ZO2PDZ2 | [U-100% 15N] | 0.8 (±0.1) mM | |
6 | phosphate buffer | natural abundance | 20 mM | |
7 | NaCl | natural abundance | 50 mM | |
8 | EDTA | natural abundance | 1 mM |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5, Details 0.8mM 15N, 13C-labeled ZO2PDZ2 domain, 20mM phosphate buffer (pH 6.5), 50mM NaCl, 1mM EDTA, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
9 | ZO2PDZ2 | [U-100% 13C; U-100% 15N] | 0.8 (±0.1) mM | |
10 | phosphate buffer | natural abundance | 20 mM | |
11 | NaCl | natural abundance | 50 mM | |
12 | EDTA | natural abundance | 1 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5, Details 0.8mM 15N, 13C-labeled ZO2PDZ2 domain, 20mM phosphate buffer (pH 6.5), 50mM NaCl, 1mM EDTA, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
13 | ZO2PDZ2 | [U-100% 13C; U-100% 15N] | 0.8 (±0.1) mM | |
14 | phosphate buffer | natural abundance | 20 mM | |
15 | NaCl | natural abundance | 50 mM | |
16 | EDTA | natural abundance | 1 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 6.5, Details 0.4mM ZO2PDZ2 domain,0.4mM 15N, 13C-labeled ZO2PDZ2 domain, 20mM phosphate buffer (pH 6.5), 50mM NaCl, 1mM EDTA, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
17 | ZO2PDZ2 | [U-100% 13C; U-100% 15N] | 0.4 (±0.05) mM | |
18 | ZO2PDZ2 | natural abundance | 0.4 (±0.05) mM | |
19 | phosphate buffer | natural abundance | 20 mM | |
20 | NaCl | natural abundance | 50 mM | |
21 | EDTA | natural abundance | 1 mM |