BMRB: 15158

Solution structure of the adhesion protein Bd37 from Babesia divergens

Authors

Auguin, D., Yang, Y., Lohr, F., Arold, S., Schetters, T., Precigout, E., Gorenflot, A., Delbecq, S., Roumestand, C.

Assembly

Bd37

Entity

1. Bd37 (polymer, Thiol state: all disulfide bound), 294 monomers, 31467.23 Da Detail

CTNLNGSQEP AAANPVVSTP GNDAQQAGTQ QGGANSKSVP EQQPQQAAGE TTATVVVKTL DVLRGELRGQ REAFLSEIIK SDGPFTILQL VGYLRVVDTD LLLKVDSTKV DEAGKKVKAY LEKIGIRGDS VEAALDNLMI KVYEITKGTV ESSAQGTDSE ELKTLLLKFS EDLKAEQELH SEAKGGEALL SSMKTQHDEL LKKFAALTPT FLTSEDISGY LTVPEYGAPM NAAKWKKVEG MIHGKLESSE VPANLKALVA ELIELREQMM DLLYGPIGHH DCAAGSGQGS SKLN

Formula weight

31467.23 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS1:SG	1:CYS282:SG

Source organism

Babesia divergens

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.7 %, Completeness: 77.1 %, Completeness (bb): 91.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	77.1 % (2539 of 3293)	77.0 % (1314 of 1706)	73.9 % (945 of 1278)	90.6 % (280 of 309)
Backbone	91.6 % (1595 of 1742)	85.2 % (514 of 603)	93.8 % (803 of 856)	98.2 % (278 of 283)
Sidechain	65.6 % (1193 of 1819)	72.5 % (800 of 1103)	56.8 % (392 of 690)	3.8 % (1 of 26)
Aromatic	39.2 % (51 of 130)	61.5 % (40 of 65)	17.2 % (11 of 64)	0.0 % (0 of 1)
Methyl	67.6 % (242 of 358)	82.7 % (148 of 179)	52.5 % (94 of 179)

1. Bd37

CTNLNGSQEP AAANPVVSTP GNDAQQAGTQ QGGANSKSVP EQQPQQAAGE TTATVVVKTL DVLRGELRGQ REAFLSEIIK SDGPFTILQL VGYLRVVDTD LLLKVDSTKV DEAGKKVKAY LEKIGIRGDS VEAALDNLMI KVYEITKGTV ESSAQGTDSE ELKTLLLKFS EDLKAEQELH SEAKGGEALL SSMKTQHDEL LKKFAALTPT FLTSEDISGY LTVPEYGAPM NAAKWKKVEG MIHGKLESSE VPANLKALVA ELIELREQMM DLLYGPIGHH DCAAGSGQGS SKLN

Show sample condition

Sample #1

Pressure 1 atm, Temperature 310 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Bd37	[U-99% 15N]		0.5 ~ 1.0 (±0.1) mM
2	phosphate buffer	natural abundance		20 mM
3	NaCl	natural abundance		50 mM

Sample #2

Pressure 1 atm, Temperature 310 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
4	Bd37	[U-99% 13C; U-99% 15N]		0.5 ~ 1.0 (±0.1) mM
5	phosphate buffer	natural abundance		20 mM
6	NaCl	natural abundance		50 mM

Sample #3

Pressure 1 atm, Temperature 310 (±0.1) K, pH 6.8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
7	Bd37	[13C]-I,V,L methyl		0.5 ~ 1.0 (±0.1) mM
8	phosphate buffer	natural abundance		20 mM
9	NaCl	natural abundance		50 mM

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Protein Blocks Logo

Calculated from 10 models in PDB: 2JO7, Strand ID: A Detail

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Release date

2008-05-05

Citation 1

1H, 15N and 13C Backbone resonance assignments of the 37 kDa surface antigen protein Bd37 from Babesia divergens
Yang, Y., Delbecq, S., Roumestand, C.
J. Biomol. NMR (2005), 31, 67-68, PubMed 15692742 , DOI 10.1007/s10858-004-6926-4 , Abstract

Show more 1 citaion

Citation 2

The solution structure of the adhesion protein Bd37 from Babesia divergens reveals structural homology with eukaryotic proteins involved in membrane trafficking
Delbecq, S., Auguin, D., Yang, Y., Lohr, F., Arold, S., Schetters, T., Precigout, E., Gorenflot, A., Roumestand, C.
J. Mol. Biol. (2008), 375, 409-424, PubMed 18035372 , DOI 10.1016/j.jmb.2007.08.019 , Abstract

Hide non-primary 1 citaion

Experiments performed 5 experiments Detail

NMR combined restraints 3 contents Detail